毒素清颗粒对细菌性肺炎老龄大鼠多器官 损伤炎性因子的影响

Effect of Dusuqing Granule: Inflammatory Factors in Multiple Organ Injury induced-by Bacterial Pneumonia in Aged Rats

摘要:

目的: 从细胞炎性因子方面揭示毒素清颗粒对克雷伯杆菌肺炎所致多器官损伤保护作用机制。 方法: 将55只老龄大鼠随机分为空白对照组、模型组、毒素清组和洛美沙星组,模型组25只,其余3组均为10只;采用气管插管法注入肺炎克雷伯杆菌制备肺炎致多器官损伤动物模型;观察肺、心、小肠等组织炎性因子白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达变化。 结果: 与空白对照组比较,模型组肺、心、肾、小肠组织TNF-α,IL-1,IL-6表达水平明显增高(P<0.01或P<0.05);外周血白细胞计数、中性粒细胞亦显著增高(P<0.01);脏器组织学发生了明显的病理改变(P<0.01或P<0.05)。与模型组比较,毒素清组肺、心、小肠、肾TNF-α,IL-1,IL-6蛋白表达明显降低(P<0.01或P<0.05);白细胞计数和中性粒细胞明显降低(P<0.01);脏器损伤程度明显减轻(P<0.01或P<0.05)。 结论: 降低组织炎性因子IL-1,IL-6,TNF-α表达水平,从而减轻脏器损伤,可能是毒素清对老年多器官损伤保护作用的主要机制之一。

Abstract:

Objective: To reveal the mechanism of the protection of Dusuqing Granule through the levels of the inflammatory factor expression in multiple organ injury caused by Klebsiella pneumonia. Method : Fifty-five male Sprague Dawley (SD) aged rats were randomlLy divided into control group (CG), model group (MG), Dusuqing group (DG) and Lomefloxacin group (LG). There were 25 rats in the model group and 10 in each of the other three groups. The models of multiple organ injury caused by pneumonia were made by injection of Klebsiella pneumoniae through the tracheal intubation. The expressions of inflammatory factors like tumor necrosis factor-α(TNF-α), interleukin-1(IL-1) and interleukin-6(IL-6) in the lungs, the theart, the kidney and the small intestine were measured. Result: The level of expressions of TNF-α, IL-1 and IL-6 in the lungs, the hear, the kedney and the small intestine in MG were significantly higher than those in CG (P<0.01 or P<0.05). The neutrophil rates and the counts of WBC in peripheral blood increased dramatictly in MG (P<0.01). The pathological changes under light microscope were obvious in the organs in MG (P<0.01 or P<0.05). Compared with MG, the levels of expressions of TNF-α, IL-1, and IL-6 in the lungs, the heart, the kidney and the small intestine in DG decreased significantly (P<0.01 or P<0.05). The neutrophil rates and the counts of WBC in peripheral blood dropped dramaticly in DG (P<0.01). The pathological injuries were light obviously in the organs in DG (P<0.01 or P<0.05). Conclusion: Low the levels of inflammatory factor expression, such as TNF-α, IL-1 and IL-6, so as to reduce organ damage, which might be one of the mechanisms of Dusuqing Granule’s protection in multiple organ injury.