通络生骨胶囊对大鼠应力缺失性骨质疏松的防治作用

Effect of Tongluo Shenggu Capsules on Osteoporosis Induced by Stress Absence

摘要:

目的: 探讨通络生骨胶囊对大鼠应力缺失性骨质疏松模型的改善作用。 方法: 40只大鼠随机分为正常对照组,模型对照组,通络生骨胶囊低(0.6 g ·kg^-1)、高剂量(1.2 g ·kg^-1)组,除正常对照组外,利用鼠尾悬吊模型制备大鼠应力缺失性骨质疏松模型,药物组在造模同时给与通络生骨胶囊。造模21 d,采血,测定血清中碱性磷酸酶、酸性磷酸酶,钙含量;处死动物,取其胫骨及股骨,切片,观察骨形态变化。 结果: 空白对照组,模型对照组,通络出骨胶囊低、高剂量组碱性磷酸酶含量分别为:430.6,355.4,485.6, 417.3 U ·L^-1;酸性磷酸酶含量分别为13.63,8.96,7.02,8.60 U ·L^-1,血钙含量分别为1.357,1.142,1.186,1.149 mmol ·L^-1;血清总钙含量分别为2.455,2.162,2.362,2.286 mmol ·L^-1;骨小梁面积分别为0.0049, 0.0033, 0.0035 mm², 成骨细胞个数分别为11.8,7, 12.5, 9.2个;骨小梁个数分别为6.1, 4.2,4.6,5.3个。与正常对照组比较,模型组碱性磷酸酶含量,血清离子钙,血清总钙浓度均显著下降,模型组骨小梁面积百分比、成骨细胞个数、骨小梁厚度及骨小梁个数均显著降低,骨小梁间距显著升高;与模型组比较,通络生骨胶囊低、高剂量组显著升高血清碱性磷酸酶含量,骨小梁面积,成骨细胞个数以及骨小梁个数,增加了骨小梁厚度,高剂量组作用更为显著。 结论: 通络生骨胶囊可用于应力缺失性骨质疏松防治。

Abstract:

Objective: To investigate the effect of Tongluo Shenggu Capsules on the osteoporosis induced by stress absence. Method: Fourty male SD rats were randomly divided into four groups: normal control, model control, high and low dose drug groups. Besides the normal control group, all animals in other three group were subjected to tail suspension test for 21 days, the animals in drug groups were administered Tongluo Shenggu Capsule. The bone metabolism markers and biomechanical parameters were determined. Reusult: Compared with the nomal control group, the alkaline phosphatase (ALP) and the concentration of calcium in serum of model group was decreased significantly, the area and thickness of bone tranecula, the amount of osteoblast were decreased obviously, compared to the model control, Tongluo Chenggu capsules could increase ALP, the area and thickness of bone tranecula and the osteoblast amount. Conclusion: Tongluo Shenggu Capsules could enhance the growth of bone and could be used to treat osteoporotic induced by stress absence.