芪参益气滴丸改善心梗模型大鼠心室重构的相关机制

Mechanisms of Qishen Yiqi Dropping Pills on Ventricular Remodeling after Myocardial Infarction of Rat Model

摘要:

目的: 探讨芪参益气滴丸改善心梗模型大鼠心室重构的相关机制。 方法: 结扎SD大鼠冠状动脉前降支建立心肌梗死模型后随机分为芪参益气滴丸药物组、卡托普利药物组、心梗对照组各16只,另设假手术组16只。芪参益气滴丸组给与38 mg ·kg^-1 ·d^-1芪参益气滴丸混悬液ig给药、卡托普利组给与10.125 mg ·kg^-1 ·d^-1卡托普利混悬液ig给药;心梗对照组给与假手术组给等量生理盐水ig。8周后观察左室舒张终末压(LVEDP)、左室收缩内压峰值(LVSP)、 左室内压上升、下降最大速率(±dp/dt_max);左心室质量(LVM)和左心质量指数(LVMI);血清脑钠肽(BNP)、血管紧张素Ⅱ(AngⅡ)、肿瘤坏死因子α(TNF-α)、内皮素1(ET-1)等指标的变化。 结果: 8周后药物组、对照组血清AngII,TNF-α,ET-1,BNP等均大于假手术组(P<0.05);但使用药物干预后的药物组血清AngⅡ,TNF-α,ET-1,BNP等低于心梗对照组(P<0.05);药物组、对照组LVEDP大于假手术组(P<0.05);LVSP,±dp/dt_max小于假手术组(P<0.05);LVM及LVMI大于假手术组(P<0.05);使用药物干预后的药物组LVM及LVMI,LVEDP等小于心梗对照组(P<0.05),LVSP,±dp/dt_max大于心梗对照组(P<0.05)。药物组中芪参益气滴丸药物组优于卡托普利药物组,但两组比较无统计学意义。 结论 :芪参益气滴丸能改善心梗模型大鼠心室重构,其相关机制与拮抗神经内分泌因子的过度激活有关。

Abstract:

Objective: To observe the effect of Qishen Yiqi Dropping Pills on ventricular remodeling and function after myocardial infarction model in rats. Method: The myocardial infarction model of SD rat was established by logating the anterior descending coronary artery and then the rats were divided into myocardial infarction control group and medicine group (n=16 in each froup). Meanwhile, a sham-operation group was build including 16 rats. The rats in medicine group were intragastrically given Qishen Yiqi Dropping Pill at the dose of 38 mg ·kg^-1 ·d^-1. The rats in the control group and the sham-operation group were intragastrically given at the same dosage of normal saline. Eight weeks later,left ventricular end-diastolic pressure,left ventricular peak pressure, the maximum change rate of left ventricular pressure rise and fall, left ventricular mass and left ventricular mass index,serum and plasma angiotensinⅡ, tumor necrosis factor α,endothelin-1,brain natriuretic peptide, etc. were tested. Result:Eight weeks later, left ventricular end-diastolic pressure of the control group was higher than that of sham-operation group(P<0.05);left intraventricular systolic pressure,the maximum change rate of left ventricular pressure rise and fall were lower than those of the sham-operation group(P<0.05);serum ICAM-1 and plasma AngiotensinⅡ, tumor necrosis factor α, endothelin-1 and brain natriuretic peptide etc were higher than those of sham-operation group(P<0.05). After drugs intervention,left ventricular mass and left ventricular mass index,left ventricular end-diastolic pressure,serum angiotensinⅡ,tumor necrosis factor α,endothelin-1and brain natriuretic peptide of the rats in the medicine group were lower than those of the control group; left intraventricular systolic pressure, the maximum change rate of left ventricular pressure rise and fall were higher than those of the control group(P<0.05). Conclusion: Qishen Yiqi Dropping Pills can improve the ventricular remodeling and function for the myocardial infarction model in rats.