香蕉皮多糖对人乳腺癌细胞增殖、克隆形成与 核因子-κB蛋白表达的影响

Influence of Polysaccharides of Banana Peel on Proliferation, Colony Formation and Expression of Nuclear Factor-κB in Human Breast Cancer Cells

摘要:

目的: 观察香蕉皮多糖对体外培养的人乳腺癌细胞增殖、克隆形成及其核因子-κB(nudear-kappa B, NF-κB)p65蛋白表达的影响。 方法: 采用溴化四甲基偶氮 法,取对数生长期人乳腺癌细胞(MCF-7),随机分为药物组与对照组,用质量浓度为0.5,1.5,2.5,3.5 g ·L^-1香蕉皮多糖作用于MCF-7细胞,分别干预24,48,72 h。通过克隆形成试验,Western blot试验,观察香蕉皮多糖对MCF-7细胞克隆形成及对MCF-7细胞NF-κB(p65)蛋白表达的影响,试验中设置药物组与对照组,药物组MCF-7细胞加入质量浓度为0.5,1.5,2.5 g ·L^-1香蕉皮多糖干预48 h。 结果: 香蕉皮多糖对MCF-7细胞的增殖有抑制作用,诱导肿瘤细胞凋亡作用与药物浓度和药物作用时间成正相关。MCF-7细胞的克隆形成率,NF-κB(p65)蛋白的表达随香蕉皮多糖浓度升高而显著下降。 结论: 香蕉皮多糖对MCF-7细胞增殖,克隆形成有抑制作用, (P<0.05)。香蕉皮多糖干预后,MCF-7细胞表达NF-κB(p65)明显减弱。其诱导MCF-7细胞凋亡作用机制可能下调NF-κB表达水平有关。

Abstract:

Objective: To study the effect of Polysaccharides of Banana Peel on proliferation, colony formation and expression of nuclear factor- kappa B(NF-κB)p65 in human breast cancer cells. Method: The MTT assay was used, and the human breast cancer cells (MCF-7) in logarithmic phase of growth were randomly divided into drug group and control group, with the concentration of 0.5,1.5,2.5,3.5 g ·L^-1 Banana Peel polysaccharides in MCF-7 cells, Intervention durations were 24, 48,72 h accordingly.The colony formation assay and the western blot technique were used to observe the effects of Polysaccharides on human breast cancer cell colony formation and expression of NF-κB(p65) in breast cancer cells. Test set of drug group and control group, drug group MCF-7 cell with the concentration of 0.5,1.5,2.5 g ·L^-1 Banana Peel polysaccharides intervention 48 h. Result: Banana Peel polysaccharides inhibited cell proliferation and induced apoptosis of tumor cells within the drug concentration and drug effects duration of studies. MCF-7 cell colony formation rate, NF-κB (p65) protein expression decreased significantly with a dose-response manner Conclusion: Banana peel polysaccharides inhibited the MCF-7 cells proliferation and colony formation (P<0.05). Polysaccharide banana peel down-regulated the expression levels of NF-κB(p65) protein in MCF-7 cells. The mechanism of MCF-7 apoptosis may be related to down-regulation of the expression levels of NF-κB(p65).