清开灵对AD小鼠学习记忆及脑组织 β-淀粉样蛋白水平的影响

Effect of Qinkailing on Learning and Memory Ability and Expression of β-amyloid Protein of Brain Tissue in Alzheimer’s Disease Model Induced by Scopolamine in Mice

摘要:

目的: 研究清开灵对阿尔茨海默病(AD)模型小鼠脑组织β-淀粉样蛋白(Aβ)水平的影响。 方法: 选用雄性KM小鼠60只,随机分成6组:正常对照组、模型对照组、清开灵低、中、高剂量(20,40,80 g·kg^-1)组和阳性对照药尼莫地平0.3 g ·kg^-1组,每组10只。ig给予等体积药物或生理盐水,每日给药1次,连续给药30 d。末次给药30 min后,东莨菪碱造模,用Morris水迷宫测试行为学指标,采用双抗体夹心法测定脑组织β-淀粉样蛋白水平。 结果: Morris水迷宫测试表明,清开灵能明显缩短模型小鼠游泳时间及游泳路程;脑组织Aβ含量测定显示清开灵能显著降低脑组织Aβ水平。 结论: 清开灵能改善造模小鼠的学习记忆功能,其机制可能与降低脑组织Aβ水平有关。

Abstract:

Objective: To study the effect of Qingkailing in model mice by measuring learning and memory ability and the expression of β-amyloid protein in brain tissue. Method :Male KM mice were randomly divided into 6 groups: Control group; Model group; L-QKL group (20 g ·kg^-1); M-QKL group(40 g ·kg^-1); H-QKL group (80 g ·kg^-1) and Nim group (0.03 g ·kg^-1). All the mice were administrated by ig with drugs or water in the same volume for 30 days. AD model of mice was established by ip scopolamine. The Morris water maze was used to observe the learning and memory ability for the model mice. Biochemical methods were used to determine the content of β-amyloid protein in brain tissue. Result: :Qingkailing could significantly improve the memory of model mice compared with the control group, Qingkailing could decrease the content of β-amyloid protein in the brain tissue obviously. Conclusion: Qingkailing could improve the ability of learning and memory of model mice, and its mechanism might involve in reducing the level of β-amyloid protein in brain tissue.