漏芦醇提取物的体外抗氧化作用

Antioxidative Effect of Ethanol Extract of Rhaponticum uniflorum

摘要:

目的: 研究祁州漏芦醇提取物的抗氧化活性。 方法: 以高铁还原法检测祁州漏芦醇提取物对肝组织总抗氧化能力的影响,以硫代巴比妥酸(TBA)法检测对肝组织的抗脂质过氧化作用。 结果: 漏芦醇提取物浓度依赖性地增高肝匀浆总抗氧化能力,同时浓度依赖性地抑制肝组织脂质过氧化作用,其对H₂O₂, ·OH和Fe²⁺诱导的肝匀浆脂质过氧化作用的半数抑制浓度(IC₅₀)分别为56.4,144.2,355.6 mg ·L^-1;对H₂O₂和Fe²诱导的肝线粒体脂质过氧化作用的IC₅₀分别为781,36.3 mg ·L^-1。 结论: 漏芦醇提取物具有体外抗氧化作用。

Abstract:

Objective: To study the antioxidative effect of ethanol extract of Rhaponticum uniflorum. Method : The effect of R.uniflorum ethanol extract on the total antioxidant capacity of liver was determined by ferric reducing method, and the anti-lipid peroxidative activity was detected by thiobarbituric acid (TBA) method. Result :R. uniflorum ethanol extract concentration-dependently elevated the total antioxidant capacity of liver homogenate, inhibited the lipid peroxidation of liver homogenate induced by H₂O₂, ·OH and Fe²⁺ with the IC₅₀s of 56.4, 144.2 and 355.6 mg ·L^-1, respectively, and inhibited the lipid peroxidation of liver mitochondria induced by H₂O₂ and Fe²⁺ with the IC₅₀s of 781 and 36.3 mg ·L^-1, respectively. Conclusion: The ethanol extract of R. uniflorum has an antioxidative effect in vitro.