组分配伍对延胡索总生物碱中主要成分体外吸收的影响

Prescription Compatibility Effect on Absorption of Major Components in Yanhusuo Extract

摘要:

目的: 建立检测M199培养液中延胡索乙素和脱氢紫堇碱的HPLC分析方法,并探讨复方给药和延胡索提取物给药对这2种成分在体外吸收的影响差异。 方法 :采用大鼠肠囊外翻法探讨不同配伍对延胡索乙素和脱氢紫堇碱吸收量的影响规律,采用HPLC-UV方法测定药液浓度,并对实验结果进行统计分析。 结果 :色谱采用SB-C₁₈反相柱,流动相为乙腈-50 mmol ·L^-1磷酸二氢纳梯度洗脱,运行27 min。延胡索乙素的线性范围0.29~46.4 mg ·L^-1;脱氢紫堇碱的线性范围0.09~22.7 mg ·L^-1,两种成分的提取回收率为95%~100%,方法的精密度、准确度和稳定性均符合要求。 结论 :本方法操作简单、专属性强、灵敏度高、准确性好,可用于延胡索乙素(THP)和脱氢紫堇碱(DHC)的肠吸收的测定。肠吸收实验结果显示复方配伍对其THP的吸收没有影响,但可以显著提高DHC的吸收。

Abstract:

An improved everted gut sac method was applied to the prescription compatibility effect on absorption of the major components in Rhizoma Coridalis extracts. With the separation and detection, tetrahydropalmatine(THP) and dehydrocorydaline(DHC) were quantified by HPLC-UV. The standard curves of THP and DHC were lineary over the concentration range of 0.29-46.4 and 0.09-22.7 g ·mL^-1 respectively. The extraction recovery of both compounds were 95%-100%. The precisions, the accuracy and the stability all met the requirements. The contents of THP did not show difference before and after compatible prescription administrated, where the DHC had a significant increase in the same process. The results revealed that DHC might lead to an increasing pharmacological effect after prescription compatibility.