补脾方药对大鼠脾细胞中PKC,Bcl-2和Bax蛋白表达的影响

Effect of Prescriptions of Reinforcing Spleen on PKC, Bcl-2 and Bax Protein Expression in Spleen in Rats

摘要:

目的 :通过观测大鼠脾细胞中蛋白激酶C(PKC),B细胞淋巴瘤/白血病-2基因(Bcl-2)和Bax蛋白表达来探讨补脾方药对脾虚证的作用机制。 方法 :无特定病原体(SPF)大鼠采用复合方法制成脾虚模型后分别以3种不同配伍补脾方药灌胃3周。采用免疫组化法测定脾细胞中PKC,Bcl-2和Bax蛋白表达。 结果: 模型组与对照组比较, Bcl-2表达减少而Bax表达增加;中药组与模型组比较,Bcl- 2表达增加而Bax表达减少;中药用药组与脾虚模型组比较,PKC表达减少( P <0.05),匀有统计学意义。 结论: 补脾方药治疗脾虚证的作用机制可能与提高脾细胞中Bcl-2表达,降低Bax和PKC表达有关。

Abstract:

Objective: To explore the change of the expression of apoptotic regulatory protein PKC, Bcl-2 and Bax in spleens of spleen deficiency rats. Method: Sixty SPF rats were divided equally into five groups.Expression of Bcl-2 and Bax in spleens was studied by immunohistochemistry method. Result: Compared to the control group, the expression of Bcl-2 was markedly lower in the model group. Compared to the model group,the level of Bcl-2 was significantly increased while levels of Bax and PKC was significantly decreased. Conclusion: Effect mechanism of Spleen-Invigorating recipe on spleen deficiency may be related to the incresed of Bcl-2 as well as the decrese of Bax and PKC.