金樱根多糖的制备及其体内抗肿瘤作用初探

Separating and Purifying Polysaccharides from Root of Cherokee Rose and its in vivo Effect of Antitumor Activity

摘要:

目的: 从金樱根中分离提取金樱根多糖,并探索其抗肿瘤活性。 方法 : 水提醇沉法提取,Sevag法除蛋白,大孔树脂脱色后获得金樱根多糖粗品。建立小鼠S180肉瘤腹水瘤模型,并将小鼠随机分为4组:生理盐水组(阴性对照组)、金樱根多糖低、中、高剂量组(50,100,200 mg ·kg^-1 )。建立移植实体瘤模型,将小鼠随机分为8组:生理盐水组(阴性对照组)、阳性对照5-Fu(5-氟脲嘧啶)组(20 mg ·kg^-1 )、金樱根多糖低、中、高剂量组(50,100,200 mg ·kg^-1 )、金樱根多糖各剂量组+5-Fu组。腹腔注射给药结束后处死小鼠,计算各用药组抑瘤率、生命延长率和白细胞数、胸腺指数、脾指数,并用EILSA法测定荷瘤小鼠血清中IL-2(白细胞介素-2)、TNF-α(肿瘤坏死因子-α)水平。 结果: 制备得到金樱根多糖粗品含糖量为85.43%。金樱根多糖50,100,200 mg ·kg^-1剂量组荷瘤小鼠生命延长率分别为3.07%±2.35%,10.11%±4.32%,34.3%±8.31%,与对照组相比,仅高剂量组有统计学意义(P<0.05)。但金樱根多糖低、中、高剂量组与5-Fu组(20 mg ·kg^-1)合用抑瘤率分别为62.74%±4.35%,67.92%±3.24%,78.79%±6.23%,明显优于单用5-Fu(P<0.05),且与单用5-Fu组相比合用能升高白细胞数、胸腺指数、脾指数及IL-2水平(P<0.05),但对TNF-α水平无影响。 结论: 金樱根多糖单用不能有效抑制小鼠S180肉瘤生长,但与5-Fu合用有明显的增效减毒作用。

Abstract:

Objective: To separate and purify polysaccharides from root of Cherokee Rose (Jinyinggen,JYG) in Chinese, and investigate its antitumor activity in vivo. Method :Water extracting and ethanol precipitating methods were applied to extract polysaccharide from root of Cherokee Rose. Sevage method was used to remove protein, and macro porous resin absorption was used for decolorization. Mice tumor model was established with transplantion of S180 solid tumor or ascites tumor. And the S180- ascites mice were divided into four groups: control(NS), JYG-Low, medium, high (50, 100, 200 mg ·kg^-1). For the mice model with transplanted S180 solid tumor, the mice were divided into eight groups: control(NS),5-Fu(20 mg ·kg^-1),JYG-Low,medium,high (50,100,200 mg ·kg^-1), JYG-Low,medium,high (50,100,200 mg ·kg^-1)+5-Fu(20 mg ·kg^-1). Intraperitoneal injection was used to be the major route of administration. The effects of different groups on the inhibitory rate, the proliferation rate,WBC,thymus index(TI) and spleen index(SI) were observed after the mice were sarcrified. Result: The sugar content of JYG polysaccharides was 85.43%.The life-prolonging rates of JYG polysaccharides (low, medium, high) were 3.07%±2.35%,10.11%±4.32%,34.3%±8.31% respectively. There was only significant differences between high dose group and control group. The antitumor effect of JYG polysaccharides-low,meddian,high doses combinated with 5-Fu were superior to 5-Fu, the inhibitory rate were 62.74%±4.35%,67.92%±3.24%,78.79%±6.23% respectively. Compared with 5-Fu alone, the drug combination could increase WBC count, TI, SI and the IL-2 level, but had no effect on TNF-α. Conclusion : The single use of JYG polysaccharides was not effect to inhibit the growth of S180 tumor, but the combination of JYG polysaccharide with 5-Fu had a coordinated effect with better efficacy and lower toxicity.