辛芷鼻敏胶囊对变应性鼻炎大鼠骨髓及外周血IL-5 及嗜酸性粒细胞的影响

Action of Xinzhi Bimin Capsule in Model of Rats with Allergic Rhinitis and Influence on levels of Interleukin-5 and Eosinophil

摘要:

目的 :探讨辛芷鼻敏胶囊对变应性鼻炎(AR)大鼠骨髓及外周血中IL-5和外周血及骨髓中嗜酸性粒细胞(EOS)的影响。 方法 :用卵清蛋白(OVA)致敏SD大鼠制成模型,分正常对照组、模型组、鼻炎康组、辛芷鼻敏胶囊组,分别ig生理盐水、鼻炎康片(3 g·kg^-1)和辛芷鼻敏胶囊(3.6 g·kg^-1),连续10 d。采用双抗体夹心酶联免疫吸附技术(ELISA)定量测定骨髓及外周血中白介素-5(IL-5)含量,骨髓和外周血涂片瑞氏染色计数EOS。 结果 :辛芷鼻敏胶囊组骨髓及外周血中IL-5,EOS比模型组有显著降低( P <0.05),且与鼻炎康组比较有统计学意义( P <0.05);辛芷鼻敏胶囊组大鼠鼻黏膜EOS计数明显低于模型组和鼻炎康组(P＜0.01,P＜0.05)。 结论 :辛芷鼻敏胶囊可以减少IL-5水平,减少嗜酸性粒细胞浸润,而起到一定的治疗作用。

Abstract:

Objective: To investigate effect of Xinzhi Bimin capsule on the model of rats with allergic rhinitis(AR) and the effect on Interleukin-5(IL-5) and eosinophil(EOS). Method :Fourty rats were randomly divided into control group,sensitized group, the positive control (Beiyankang, 3 g ·kg^-1)and experimental groups(3.6 g ·kg^-1).The rats were sensitized by ovalbumin. Result : IL-5 content in bone marrow of the experimental group was significantly lower than those of the model group and the positive control group(P<0.05); IL-5 content of blood serum in the experimental group was significantly lower than those of the model group and the positive control group(P<0.05); the numbers of EOS, compared with the control group and the model group,there were significant difference(P<0.05). Conclusion : Xinzhi Bimin capsule can reduce IL-5 content of bone marrow and in serum,and relieve EOS infiltration.