大蒜超临界萃取物体外抗真菌活性研究

Antifungal Action in vitro of Extracts from Garlic by Supercritical Fluid Extraction

摘要:

目的: 研究大蒜超临界萃取物体外抗真菌活性。 方法: 采用培养基药物浓度稀释法测试大蒜超临界萃取物对15株皮肤癣菌(上海株:断发毛癣菌、大脑状毛癣菌、石膏样小孢子菌、红色毛癣菌、石膏样毛癣菌、羊毛状小孢子菌、絮状表皮癣菌;武汉株:红色毛癣菌、须毛癣菌、石膏样毛癣菌、无色红毛癣菌、犬小孢子菌、羊毛状小孢子菌、絮状毛癣菌、白色念珠菌)的最小抑菌浓度(MIC)及最小杀菌浓度(MBC)。 结果: 大蒜超临界萃取物对上海株(断发毛癣菌)、武汉株(须毛癣菌、羊毛状小孢子菌及无色红毛癣菌)4株菌株的MIC为3.13 mg ·L^-1,对上海株(石膏样小孢子菌、羊毛状小孢子菌)及武汉株(羊毛状小孢子菌)3株菌株的MIC为1.56 mg ·L^-1,对上海株(大脑状毛癣菌、红色毛癣菌、石膏样毛癣菌)、武汉株(红色毛癣菌、石膏样毛癣菌、犬小孢子菌及白色念珠菌)7株菌株的MIC均小于0.78 mg ·L^-1。杀菌实验结果显示对上海株(石膏样小孢子菌、絮状表皮癣菌)、武汉株(猴毛癣菌、絮状表皮癣菌)4株菌株的MBC为6.25 mg ·L^-1,对上海株(断发毛癣菌、羊毛状小孢子菌)、武汉株(红色毛癣菌、无色红色毛癣菌及犬小孢子菌)5株菌株的MBC为3.13 mg ·L^-1,对大脑状毛癣菌上海株的MBC为1.56 mg ·L^-1,对上海株(红色毛癣菌、石膏样毛癣菌)、武汉株(石膏样毛癣菌、白色念珠菌)4株菌株的MBC均小于0.78 mg ·L^-1。 结论: 大蒜超临界萃取物对15株皮肤癣菌有较强的体外抗真菌活性。

Abstract:

Objective: To study antifungal action in vitro of extracts from Garlic by supercritical fluid extraction (SFE). Method: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of SFE extract of Garlic against 15 strains dermatophytes were determined by media dilution method. Result: MIC of SFE of garlic to Shanghai strain (Trichophyton tousurans) and Wuhan strains (Trichophyton memtigrophytes, Wool microsporum canis, Achromatism trichophyton rubrum) were 3.13 mg ·L^-1, MIC to Shanghai strains(Plaster microsporum canis, Wool microsporum canis)and Wuhan strain(Wool microsporum canis) were 1.56 mg ·L^-1, MIC of SFE to Shanghai strains (Trichophyton cerebriforme, Trichophyton rubrum, Trichophyton gypserum) and Wuhan strains (Trichophyton gypserum, Trichophyton rubrum, Microsporum canis, Candida albicans ) were less than 0.78 mg ·L^-1. MBC values were different. Apart from MBC of SFE of garlic to Shanghai strains(Plaster microsporum canis, Epidermophyton floccosum)and Wuhan strains (Trichophyton mentagroph, Epidermophyton floccosum) were 6.25 mg ·L^-1, MBC to Shanghai strains (Trichophyton tousurans, Wool microsporum canis) and Wuhan strains (Trichophyton rubrum, Achromatism trichophyton rubrum, Microsporum canis) were 3.13 mg ·L^-1, MBC to Trichophyton cerebriforme(Shanghai strain)was 1.56 mg ·L^-1, MBC to Shanghai strains (Trichophyton rubrum, Trichophyton gypserum) and Wuhan strains (Trichophyton gypserum,Candida albicans) were less than 0.78 mg ·L^-1. Conclusion: The SFE extract of Garlic showed strong antifungal effect in vitro.