大孔吸附树脂对牛蒡子中牛蒡苷的纯化工艺研究

Study on Purification of Arctiin from Arctium lappa by Macroporous Resin

摘要:

目的: 优选出牛蒡子药材醇提液经树脂纯化的最佳工艺条件。 方法: 用高效液相色谱法测定牛蒡苷的含量,用树脂吸附率、解析率为指标考查大孔吸附树脂对牛蒡苷的吸附行为。 结果: D101树脂对牛蒡苷提取液纯化最优,上样液牛蒡苷浓度为28.69 g ·L^-1,洗脱流速3 BV ·h^-1,用蒸馏水5 BV洗脱,然后用30%乙醇10 BV洗脱,收集洗脱液,浓缩、干燥,即得到牛蒡苷总苷。 结论: 该方法简单、可行, 能够用来富集纯化牛蒡子中牛蒡苷。

Abstract:

Objective: To establish an appropriate and technical process for the purification of Arctium lappa by macroporous resins. Method: The HPLC was used to analyze the content of arctiin in A.lappa, and the adsorption behavior of macroporous resins to arctiin in A.lappa was examined for the adsorption rate and desorption rate. Result: The D101 resin was the suitable for enriching arctiin. The sample concentration of arctiin was 28.69 g ·L^-1 with the elution flow rate of 3 BV ·h^-1. 5 BV water and 10 BV 30% ethanol were used as eluant of arcttin, respectively. Portions of 30% ethanol were collected, concentrated, and dried. Conclusion: This method is simple and feasible with good effect of enrichment in arctiin.