伸筋草总生物碱提取工艺

Study on Extraction of Total Alkaloids from Lycopodium japonicum

摘要:

目的: 对伸筋草总生物碱提取工艺进行研究,为工业化生产奠定基础。 方法: 以伸筋草中总生物碱为指标,建立分析方法,采用正交设计方法考察盐酸水浸提的最佳工艺和使用阳离子交换树脂工艺纯化总生物碱。 结果: 盐酸水浸提的最佳工艺为加入15倍1%盐酸,浸提3次,每次24 h,滤过,合并酸水液,以3 BV ·h^-1流速上001×7阳离子交换树脂柱,10倍柱体积8%氨水乙醇洗树脂柱。 结论: 该方法工艺较简单,成本低,产率高,可作为伸筋草总生物碱的工艺。

Abstract:

Objective: To study the extraction technological parameters of the total alkaloids from Lycopodium japonicum. Method: The orthogonal test was used to optimize extracting conditions in the process of acid water extraction. Then the optimized conditions for purification using cation exchange resin were investigated. Result: The optimized conditions in the process of acid water extraction were 0.5% hydrochloric acid as much as 15 times of the medicine amount for 24 h and three times. Then the extraction of acid water was purified with a column of macroporous cation exchange resin 001×7 at 3 BV ·h^-1 of flow rate, then eluted with 10 BV of 8% aqueous ammonia ethanol. Conclusion: This technology is rapid and accurate for the purification of total alkaloids from L.japonicum.