以高尿酸血症小鼠模型优选痛风定颗粒的提取工艺

Screen Extraction Process of Tongfengding Granules by Mouse Model of Hyperuricemia

摘要:

目的: 通过高尿酸血症小鼠模型对痛风定颗粒的不同提取工艺进行优选。 方法 :通过灌胃给予尿酸酶抑制剂氧嗪酸钾制造高尿酸(UA)血症小鼠模型,分别给予痛风定颗粒处方经水提取、水提醇沉、70%乙醇提取所得浸膏,紫外分光光度法测定各组的血尿酸(SUA)浓度。 结果 :与正常组相比,模型组小鼠血尿酸水平显著升高(P﹤0.001);与模型组相比,水提取所得浸膏能够显著降低高尿酸血症小鼠的血尿酸水平(P﹤0.01);水提醇沉上清液、醇沉部分对高尿酸血症小鼠的血尿酸水平无显著性影响; 70%乙醇提取浸膏能够显著升高模型组小鼠的血尿酸水平(P﹤0.001)。 结论: 来源于临床的水提取工艺为痛风定颗粒的最佳提取工艺。

Abstract:

Objective: Screen the extraction process of Tongfengding granules by the mouse model of hyperuricemia. Method: Given by gavage uricase inhibitor potassium oxide hydrochloride manufacture of high uric acid (UA) mouse model of hyperlipidemia, the mice were fed grain with water extraction, water extraction and alcohol precipitation, 70% ethanol extracts derived from extracts of Tongfending graules,and then the concentrations of uric acid in the blood of each group (SUA) were determined by UV spectrophotometer. Result: Compared with normal group, the serum uric acid levels in model group mice were significantly higher (P<0.001);compared with model group, the water extract obtained extract could significantly reduce the high uric acid levels in mice serum uric acid (P<0.01); supernatant and precipitation part in water extraction and alcohol precipitation had no significant impact on serum uric acid levels of high serum uric acid mice; 70% ethanol extracts could significantly increase serum uric acid levels in model group mice (P<0.001). Conclusion: Water extraction from the clinical setting for Tongfengding graules is the best particle extraction.