延胡索总生物碱纯化工艺

Studies on Purification Technology of Total Fumaric Alkaloids

摘要:

目的: 研究采用阳离子交换树脂法富集延胡索生物碱的工艺参数。 方法: 以总生物碱交换量为考察指标,经阳离子交换树脂法富集后,采用紫外分光光度法测定。 结果: 纯化工艺条件为药材-浓缩液=1 ∶5做为上柱液,药材比树脂为1 ∶1,流速2 BV ·h^-1,径高比约为1 ∶10,5 BV的纯化水2 BV ·h^-1水洗除杂, 4 BV的含5%氨水的70%乙醇2 BV ·h^-1洗脱树脂柱。 结论: 该法富集纯化生物碱稳定可行。

Abstract:

Objective: To study the method of enrichment of fumaric alkaloids by using cation resin. Method: Ultraviolet spectrophotometry was used to determine total fumaric alkaloid with exchange capacity as index. Result: The optimum purification condition was as follows: herbs: concentrate solution was about 1 ∶5, herbs: cation resin was about 1 ∶1; flow rate was 2 BV ·h^-1, diameter:high of cation resin column was about 1 ∶10; 5 BV of purified water eluted resin column to removed impurities, flow rate was 2 BV ·h^-1, 4 BV of 70% ethanol as eluent consisted of 5% ammonia, 2 BV ·h^-1 flow rate washing resin column. Conclusion: The optimum condition was stable and can be used for purification of alkaloids.