毛细管气相色谱法测定红药贴膏中4种活性成分的含量

Determination of Four Active Components in Hongyao Strapping by Capillary Gas Chromatography

摘要:

目的: 建立毛细管气相色谱法同时测定红药贴膏中樟脑、薄荷脑、冰片和水杨酸甲酯的含量。 方法: 采用毛细管气相色谱法。色谱柱为PEG-20M石英毛细管柱(0.25 mm×0.25 μm×30 m),氢火焰离子检测器,检测器和气化室的温度均为200 ℃,柱温160 ℃,氮气为载气,正辛醇为内标物,用挥发油测定器蒸馏制备供试品溶液。 结果: 在该色谱条件下,樟脑、薄荷脑、冰片和水杨酸甲酯分离度和线性关系良好,回收率分别为99.8%, 100.3%, 99.5%, 99.7%。 结论: 该方法简便、准确、专属性强、重复性好,可用于红药贴膏的质量控制。

Abstract:

Objective: To establish a method for determination of camphor, menthol, borneol and methylsalicylate in Hongyao strapping. Method: Capillary gas chromatography was adopted. The chromatography separation was carried out with PEG-20M capillary column(0.25 μm×0.25 mm×30 m), using a flame ionization detector. The injector and detector temperature were set at 200 ℃. The column temperature was 160 ℃. n-Octanol was used as internal standard. The sample solution was prepared by distillation with essential oil collector. Result: The resolution and linearity of camphor, menthol, borneol and methylsalicylate were good under the chromatographic conditions. The average recoveries of camphor, menthol, borneol and methylsalicylate were 99.8%, 100.3%, 99.5%, 99.7%. Conclusion: This method is simple, sensitive, accurate and has good repeatability for controlling the quality of camphor, menthol, borneol and methylsalicylate in Hongyao strapping.