HPCE测定脑力宝丸中远志皂苷元和远志酸

Determination of Senegenin and Polygalacic Acid in Naolibao Pill by HPCE

摘要:

目的: 建立测定脑力宝丸中远志皂苷元和远志酸含量的高效毛细管电泳法。 方法: 未涂层毛细管柱(75 μm×57 cm,有效长度50 cm);缓冲液20 mmol ·L^-1硼砂与20%甲醇;分离电压25 kV;检测波长210 nm。 结果: 远志皂苷元、远志酸的线性范围分别为13.25~212 mg ·L^-1(r=0.997 1),12.5~200 mg ·L^-1 (r=0.997 7)。脑力宝丸中远志皂苷元、远志酸的平均回收率分别为100.5%,100.6%。 结论: 该法简便,准确、重复性好,可用于脑力宝丸的质量控制。

Abstract:

Objective: To establish a determination method for senegenin and polygalacic acid in Naolibao pills by capillary electrophoresis. Method: The separation was carried out in an uncoated fused silica capillary (75 μm×50 cm). The running voltage was 25 kV. The buffer was 20 mmol ·L^-1 Na₂B₄O₇ containing 20% methanol. The wavelength was at 210 nm. Result: senegenin was linear in the range of 13.25-212 mg ·L^-1(r=0.997 1) and polygalacic acid was linear in the range of 12.5-200 mg ·L^-1 (r=0.997 7). The average recoveries of senegenin and polygalacic acid in Naolibao pills were 100.5% and 100.6% respectively. Conclusion: This method is proved to be simple, rapid and accurate, and can be used as a new method for the quality control of Nailibao pills.