不同炮制方法对吴茱萸中3种指标性成分含量的影响研究

Effect of Different Processing Methods on Three Components in Evodia rutaecarpa

摘要:

目的: 比较吴茱萸生品及不同炮制品中吴茱萸内酯、吴茱萸碱及吴茱萸次碱的含量差异。 方法: 采用Agilent C₁₈色谱柱(4.6 mm ×250 mm, 5 μm),以乙腈-水(45 ∶55)溶剂系统为流动相,检测波长为225 nm。 结果: 吴茱萸生品及不同炮制品中三种成分的含量存在明显差异。 结论: 不同炮制品中吴茱萸内酯、吴茱萸碱及吴茱萸次碱的含量不同,为临床用药提供科学的依据。

Abstract:

Objective: An HPLC method was established for the determination of evodin, evodiamine and rutaecarpine in Evodia rutaecarpa and the processed product. Method: RP-HPLC method was established with an Agilent C₁₈ column (4.6 mm ×250 mm, 5 μm). The mobile phase was acetonitrile and water (45 ∶55). The detective wavelength was at 225 nm. Result: There were obvious differences of the content of three components in E. rutaecarpa and the processed product. Conclusion: The different processing methods have certain effect on the content of evodin, evodiamine and rutaecarpine. It can provide evidence for the clinical use.