赶山鞭中金丝桃苷的HPLC测定

Determination Content of Hyperin in Hypericum attenuatum Choisy 3

摘要:

目的: 建立赶山鞭中金丝桃苷的含量测定方法。 方法: 采用VP-ODS C₁₈ (4.6 mm×150 mm,5 μm)色谱柱,以乙腈-0.1%磷酸溶液(14 ∶86)为流动相,流速1.0 mL ·min^-1,检测波长为360 nm为HPLC检测条件测量7批赶山鞭含量。 结果: 赶山鞭中的金丝桃苷含量侧定方法在0.099 8~0.998 0时进样量与峰面积的线性关系良好(r=0.999 8),平均回收率为100.11%,RSD 1.20%。 结论: 所建立的方法准确、灵敏、重复性好,可用于赶山鞭的质量控制。

Abstract:

Objective: To establish the determination method of hyperin content in Hypericum attenuatum Choisy. Method: VP-ODS C_l8(4.6 mm×150 mm, 5 μm)colmnn was used,the mobile phase was acetonitrile-0.1% phosphoric acid water(16 ∶84);with the flow rate as 1.0 mL ·min^-1, the detection wavelength was at 360 nm. Result: The linear relation of hyperin was good in the range from 0.099 8 to 0.998 0 μg.The average recovery was 100.11%,RSD 1.20%. Conclusion :The method established is accurate,sensitive and good in reproducibility.It can be applied to the qualitycontrol of H.attenuatum