白木香果皮提取物的抗菌活性

Study on Antimicrobial Activity of Aquilaria sinensis Peel Extract

摘要:

目的: 为开发利用白木香果皮资源,对其提取物进行抗菌活性评价。 方法: 用三氯甲烷对白木香果皮进行提取,提取物分别采用滤纸片法和生长速率法对不同细菌和真菌进行抗菌活性测定,并采用连续稀释法测定其最低抑菌浓度。 结果: 白木香果皮提取物对金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌有显著抑制作用,最小的抑菌浓度分别为6.25,625,12.5 g ·L^-1, 对大肠杆菌则没有抑制效果;对绿色木霉、黑曲霉、黄曲霉也有明显的抑制作用,最小的抑菌浓度分别为6.25,6.25,12.5 g ·L^-1。 结论: 白木香果皮三氯甲烷提取物具有显著的抗菌活性,值得开发利用。

Abstract:

Objective: To study antimicrobial acitvity of Aquilaria sinensis peel extract. Method: The chloroform extract of A. sinensis peel was investigated for antimicrobial activity against the tested bacteria and fungi using filterpaper disc method and growth rate method respectively and their minimum inhibitory concentrations (MICs) were determined using serial dilution method. Result: The extract displayed significant antibacterial and antifungal activities against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa with MIC values of 6.25,6.25,12.5 g ·L^-1 and against Trichoderma roseum, Aspergillus niger and A.flavus with MIC values of 6.25,6.25,12.5 g ·L^-1. Conclusion: The chloroform extract of A. sinensis peel exhibited antimicrobial activity which is worthy of exploitation.