冬凌草植株再生过程中冬凌草甲素的积累机制

Study on Accumulation Mechanism of Oridonin During Plant Regeneration in Rabdosia rubescens

摘要:

目的: 探讨冬凌草植株再生过程中培养物的生理特性与冬凌草甲素积累之间的机制。 方法: 采用紫外分光光度法、高效液相法测定不同时期培养物内叶绿素、可溶性糖、可溶性蛋白、丙酮酸、冬凌草甲素的含量。 结果: 培养物内叶绿素、丙酮酸、可溶性蛋白含量与冬凌草甲素含量呈正相关,相关系数分别为0.781,0.897,0.866;而可溶性糖含量与冬凌草甲素含量呈负相关,相关系数为-0.515。 结论: 冬凌草培养物内叶绿素、丙酮酸的含量、可溶性蛋白与冬凌草甲素积累有相互促进的关系,而可溶性糖变化主要与培养物细胞的生长、发育关系密切。

Abstract:

Objective: Changes of chlorophyll, soluble sugar, soluble protein, during the plant regeneration in Rabdosia rubescens werestudied to explore the correlation between the accumulation of the active components and physiological characteristics of cultural materials of R. rubescens. Method: UV spectrophotometry and high performance liquid chromatography were used to determine the content of chlorophyll, soluble sugar, soluble protein in the cultural materials of different stage. Result: The content of chlorophyll, soluble protein and acetic acid was positively correlated with the oridonin, while the soluble sugar had a negative correlation with the oridonin. Conclusion: The content of chlorophyll, soluble protein in the cultural materials had a mutual promotion relationship with the oridonin, and the levels of soluble sugar were closely related to the growth speed and developmental phase of the cultural materials.