黄花蒿发根的生长及部分代谢成分的特征研究

Characterization on Growth and Some Secondary Metabolites of Artemisia annua Roots

摘要:

目的: 研究黄花蒿发根的生长形态和部分次生代谢物特征。 方法: 比较发根和未转化根的形态差异,测定生长调节剂如萘乙酸(NAA)和赤霉素(GA₃)对根生长的影响,分析了发根和未转化根的部分次生代谢物的含量特征。 结果: 低浓度NAA(0.01~0.05 mg ·L^-1)能明显促进发根侧根和次侧根的形成,对未转化根则无显著作用,高浓度NAA(0.1 mg ·L^-1)抑制发根的生长,而对未转化根的生长起促进作用。未转化根中不含有青蒿素,而在发根中检测到较高含量的青蒿素(0.64 mg ·g^-1),未转化根中青蒿乙素含量较高(1.24 mg ·g^-1)。在未转化根和发根中,青蒿黄酮chrysoplenetin含量相当,而发根中另一种黄酮化合物artemitin含量(0.76 mg ·g^-1)是未转化根的3.5倍。 结论: 黄花蒿发根具有较独特的生长和次生代谢特征。

Abstract:

Objective: The characterization on growth and some secondary metabolites of Artemisia annua hairy roots were investigated. Method: We investigated the effects of plant growth regulators such as NAA and GA₃ on the hairy roots and untransformed roots. The main components of some secondary metabolites were tested in the hairy roots. Result: NAA at lower concentration (0.01-0.05 mg ·L^-1) could promote formation of lateral and branch roots and had no effect on untransformed roots. High concentrated NAA (0.1 mg ·L^-1) inhibited the growth of hairy roots but stimulated the growth of untransformed roots. Artemisinin was not detected in untransformed roots, but at 0.64 mg ·g^-1 in hairy roots. To the flavonoids in A. annua, chrysoplenetin was found in both roots, but there was a higher content of arteannuin (0.76 mg ·g^-1) in hairy roots, 3.5 folds of that in untransformed roots. Conclusion: A. annua hairy roots have a unique characterization on growth and secondary metabolites.