复方巴戟天合剂对激素性股骨头坏死大鼠血管内皮生长因子表达的影响

Effect of Complex Bajitian Mixture on Expression of Vascular Endothelial Growth Factor in Rat with Steroid-induced Osteonecrosis of Femoral Head

摘要:

目的: 探讨复方巴戟天合剂对激素性股骨头缺血性坏死大鼠血管内皮生长因子(vascular endothelial growth factor, VEGF mRNA)表达的影响。 方法: SD大白鼠72只,随机分成正常组、模型组、对照组、治疗组。除正常组外,第1~4周各组均每周2次im醋酸强的松龙24.5 mg ·kg^-1,第4~8周每周1次im醋酸强的松龙24.5 mg ·kg^-1,建立大鼠激素性股骨头缺血性坏死模型,每日给药1次,于实验后第4,8,12周分3批处死动物,逆转录-聚合酶链反应(RT-PCR)检测VEGF mRNA的表达。 结果: 模型组造模第4,8周时VEGF mRNA的表达逐渐减弱,第12周稍有升高,但仍较正常组低(P<0.05);治疗组在各时期与模型组比较均有升高(P<0.05),治疗第12周与第8周比较有显著性差异(P<0.05)。 结论: 复方巴戟天合剂可增加股骨头内VEGF mRNA的表达,可起到与仙灵骨葆胶囊相近的治疗效果。

Abstract:

Objective: To investigate the effect of Complex Bajitian mixture on the expression of VEGF mRNA in SD rats with steroid-induced osteonecrosis of the femoral head. Method: Seventy-two adult SD rats were randomized into four groups: normal control group, model group, positive control group and therapeutic group. Rat model of osteonecrosis of femoral head was established by injecting glucocorticoid. Each group was given drugs by gastric infusion one time a day. The rats were killed in the fourth, eighth and twelfth week respectively. The expression of VEGF mRNA was evaluated by RT-PCR. Result: The expression of VEGF mRNA was increased significantly after treatment on the 12^th week compared to the 4^th and 8^th week (P<0.01). Conclusion: Complex Bajitian mixture can up-regulate the expression of VEGF mRNA. The therapeutic effect is close with the Xianling Gubao capsule.