维药菊苣提取物对小鼠急性酒精性肝损伤的保护作用

Hepatoprotective Effects of Cichorium glandulosum Extracts on Alcohol-induced Acute Liver Injury in Mice

摘要:

目的: 研究维药菊苣提取物(CG-Ⅰ,CG-Ⅱ)对小鼠急性酒精性肝损伤的保护作用。 方法: 灌胃35度二锅头白酒16 mL ·kg^-1造酒精性肝损伤模型,观察肝脏、脾脏系数,血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、胆固醇(CHO)、甘油三酯(TG)、肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、一氧化氮(NO)的水平和肝脏病理组织学变化。 结果: CG-Ⅰ,Ⅱ各剂量组均可降低小鼠脾脏系数和血清ALT,TG活性,升高肝组织SOD,GSH-Px活性,并降低肝组织MDA和NO含量(P<0.05);CG-Ⅰ(1.0,2.0 g ·kg^-1)和CG-Ⅱ(1.2,2.4 g ·kg^-1)可以降低肝脏系数(P<0.05);CG-Ⅰ(1.0 g ·kg^-1)和CG-Ⅱ(1.2 g ·kg^-1)可降低小鼠血清AST活性(P<0.05),降低小鼠血清CHO活性(P<0.05);CG-Ⅰ,Ⅱ均可不同程度地改善肝脏病理组织性损伤。 结论: CG-Ⅰ,CG-Ⅱ对小鼠急性酒精性肝损伤具有保护作用。

Abstract:

Objective: To investigate the hepatoprotective effects of Cichorium glandulosum extracts (CG-Ⅰand CG-Ⅱ) against alcohol-induced acute liver injury in mice. Method: The acute liver injury animal model was established in mice by alcohol gavage (35% at 16 mL ·kg^-1). The liver and spleen indexes, alanine aminotransferase (ALT), activities of serumaspartate amiontransferase (AST), cholesterol (CHO), triglycerides(TG), activities of superoxidedismutase (SOD) and lutathione peroxidase(GSH-Px), contents of malondialdehyde (MDA) and nitric oxide (NO) in liver homogenate, as well as the histopathological changes were all studied. Result: CG-I and CG-Ⅱsignificantly reduced the spleenindex and activities of serum ALT and TG, increased the activities of liver SOD and GSH-Px, lowered the contents of MDA and NO (P<0.05 or P<0.01). Specifically, CG-I (1.0,2.0 g ·kg^-1) and CG-Ⅱ(1.2,2.4 g ·kg^-1) decreased the liver index (P<0.05); CG-I(1.0 g ·kg^-1) and CG-Ⅱ(1.2 g ·kg^-1) decreased the activity of serum AST (P<0.05); CG-Ⅰ(1.0 g ·kg^-1) and CG-Ⅱ(2.4 g ·kg^-1) decreased the activity of serum CHO (P<0.05 or P<0.01); CG-Ⅰand CG-Ⅱ improved liver injuries. Conclusion: CG-I and CG-Ⅱ showed protective effects against acute liver injury induced by alcohol in mice.