丹参注射液对老龄大鼠血液流变学指标和红细胞膜胆固醇的影响

Effect of Danshen Injection on Hemarheological Indexes and Cholesterol in Erythrocyte Membrane in Aged Rats

摘要:

目的: 从血液流变学和红细胞膜胆固醇的变化探讨丹参注射液改善老龄大鼠红细胞变形性的作用机制。 方法: 70~72周龄Wistar大鼠30只,雌雄各半,随机分为丹参注射液高剂量组(4.4 g生药/kg)、低剂量组(1.1 g生药/kg)和模型组。另外10只轻龄大鼠作为轻龄对照组,ip给药14 d后,取血测定全血黏度、血浆黏度和红细胞变性指数等指标,并用高效液相色谱法测定红细胞膜胆固醇含量。 结果: 与模型组相比,丹参注射液组能够显著降低老龄大鼠的全血黏度(P<0.01)、血浆黏度(P<0.05)以及红细胞膜上胆固醇含量(P<0.01);并能明显提高红细胞变形能力(P<0.01)。 结论: 丹参注射液可能通过减少红细胞膜上的胆固醇含量,影响红细胞膜上脂质代谢,是提高红细胞膜变形性,改善血液黏度的机制之一。

Abstract:

Objective: To investigate the action mechanism of Danshen injection on red blood cell deformability in aged rats. And the change in hemorheological indexes and cholesterol in erythrocyte membrane was studied. Method: Thirty Wistar rats (70-72 weeks old) were randomly assigned to Danshen injection high-dose group (4.4 g ·kg^-1), low-dose group (1.1 g ·kg^-1) and model group with 10 rats in each group. Another ten younger Wistar rats were as young control group. The related indexes of hemorheology, such as the whole blood viscosity, plasma viscosity and the red blood cell deformability, were determined while the contents of cholesterol in erythrocyte membrane were determined by HPLC after giving medicine 14 days by intraperitoneal injection. Result: Compared with the model group, the Danshen injection group was effective. The whole blood viscosity (P<0.01)、plasma viscosity (P<0.05) and the content of cholesterol in erythrocyte membrane (P<0.01) were decreased. And the deformability of erythrocyte (P<0.01) was increased. Conclusion: Danshen injection can decrease the content of cholesterol in erythrocyte membrane and affect the metabolism of lipid which maybe one of the mechanism of increasing deformability of erythrocyte and improving the blood viscosity.