莪术对糖尿病肾病大鼠的保护作用

Reno-protective Effects of Curcuma wenyujin on Diabetic Nephropathy in Rats

摘要:

目的: 探讨莪术(Curcuma wenyujin)对糖尿病肾病大鼠的保护作用。 方法: Wistar大鼠40只,分为假手术组、模型组和莪术治疗组,利用肾切除和链脲佐菌素(streptozotocin,STZ,45 mg ·kg^-1ip)诱导糖尿病大鼠模型并用莪术进行干预(3 g ·kg^-1 ·d^-1 ig),观察肾脏病理学变化,检测血糖(glucemia,Glu) 总胆固醇(total cholesteral,TC)、血肌酐(blood serum creatinine,Scr)、尿微量白蛋白及结缔组织生长因子(connective tissue growth factor, CTGF)在肾脏的表达变化。 结果: 模型组较假手术组Glu,TC,尿白蛋白排泄率明显增高(P<0.01),CTGF在肾组织中的表达显著增加(P<0.01),肾脏病理变化显著;而莪术治疗组上述指标较模型组显著减少(P<0.01),CTGF在肾组织中的表达显著降低(P<0.01),肾脏病理变化有明显改善。 结论: 莪术对糖尿病大鼠的肾脏有保护作用,可能与其抑制CTGF在肾组织中的表达有关。

Abstract:

Objective: To investigate the renal protective effect of Curcuma wenyujin on the kidney in diabetic rats. Method: Fouty Wistar rats were divided into sham operation group, model group and C.wenyujin-treatment group. Nephrectomy and streptozocin(STZ,45 mg ·kg^-1ip) were used to induce diabetic nephropathy and treated with C.wenyujin(3 g ·kg^-1 ·d^-1 ig).Blood glucose(Glu),total cholesterol(TC),serum creatinine clearance,amount of urinary albumin, connective tissue growth factor (CTGF) protein and pathologic change of kidney tissue were observed. Result: The amount of Glu, TC, urinary albumin and renal protein expression of CTGF were markedly increased in model group compared with those in normal group(P<0.01). Pathologic changes of kidney tissue presented further development. Treatment with C.wenyujin could significantly decrease the above indexes compared with those in diabetic model group(P<0.01),and pathologic changes of kidney tissue were significantly improved. Conclusion: C.wenyujin has reno-protective effects on diabetic nephropathy in rats, and these effects may be correlated with its inhibition on over-expression of CTGF in kidney.