川芎嗪注射液对心梗后大鼠缺血心肌血管新生及VEGF-mRNA表达的影响

Effects of Tetramethylpyrazine Injection on Angiogenesis and Expression of VEGF mRNA in Ischemic Myocardium of Rats with Myocardial Infarction

摘要:

目的: 观察川芎嗪注射液对心肌梗死后大鼠缺血心肌血管新生和缺血心肌血管内皮生长因子mRNA(VEGF mRNA)表达的影响。 方法: 结扎大鼠左冠状动脉,造成急性心肌梗死动物模型,随机分为川芎嗪注射液组20 mg ·kg^-1,ip,麝香保心丸组(阳性对照)30 mg ·kg^-1,ig,模型组,并设假手术组。6周后检测各组大鼠缺血心肌中微血管密度(MVD)及VEGFmRNA的表达及其灰度值。 结果: 模型组、麝香保心丸组及川芎嗪注射液组大鼠心肌梗死边缘区MVD较假手术组明显增多(P<0.05);川芎嗪注射液组较模型组明显增加(P<0.05),与麝香保心丸组作用相似。模型组VEGFmRNA表达及其灰度值高于假手术组(P<0.05);川芎嗪注射液组明显高于模型组(P<0.05),低于麝香保心丸组,但无统计学差异。 结论: 川芎嗪注射液可促进心肌梗死后大鼠缺血心肌血管新生,其机制可能与促进VEGFFmRNA的表达有关。

Abstract:

Objective: To observe the effects of tetramethylpyrazine injection on the angiogenesis and expression of vascular endothelial growth factor(VEGF)mRNA in ischemic myocardium of the rats with myocardial infarction(MI). Method :The left coronary artery of male Wistar rat was ligated to make the model of acute myocardial infarction.All rats were randomly divided into Tetramethylpyrazine injection group,Shexiang Baoxin Pills group(positive control group),model group and sham operation group. Microvasscular vascular density(MVD) and VEGF mRNA in ischemic myocardium of rats with MI were detected six weeks later. Result :MVC and MVD in ischemic myocardium of rats with MI in modle group, tetramethylpyrazine injection group and Shexiang Baoxin Pills group were higher than that in sham operation group.In tetramethylpyrazine injection group they were the same as Shexiang Baoxin Pills group,which were higher than that in modle group.The expression of VEGFmRNA in modle group were elevated compared with that in sham operation group.It was significantly elevated in tetramethylpyrazine injection group compared with that in modle group and lower than that in Shexiang Baoxin Pills group. Conclusion :tetramethylpyrazine injection may promote angiogenesis in ischemic myocardium of the rats with MI.One of the mechanisms might be that tetramethylpyrazine injection could promot the expression of VEGFmRNA in ischemic myocardium of the rats with MI.