补阳还五汤干预代谢综合征血管性血友病因子的研究

Experimental Study of Intervention of Buyang Huanwu Decoction on Metabolic Syndrome and von willebrand Factor

摘要:

目的: 观察补阳还五汤干预代谢综合征(MetS)模型血管性血友病因子(vWF)的作用和初步探讨其机制。 方法: 52只雄性Wistar大鼠随机分为正常、模型对照、补阳还五汤、补阳还五汤去黄芪方4组,采用高糖脂餐加链脲佐菌素建立MetS模型,补阳还五汤组按7.02 g ·kg^-1 ig,1次/d给药,补阳还五汤去黄芪方组按1.07 g ·kg^-1 ig,1次/d给药,造模和给药同时进行并持续8周,观察补阳还五汤及其去黄芪方对空腹血糖(FBG),血清胰岛素(Ins),稳态模型评价胰岛素抵抗(HOMA-IR)和血清vWF水平的影响。 结果: 模型组Ins,HOMA-IR,vWF较正常组明显升高(P<0.05);与模型组比较,补阳还五汤组的Ins,HOMA-IR,vWF明显降低(P<0.05);补阳还五汤去黄芪方组Ins,HOMA-IR与模型组比较无统计学差异,FBG明显升高(P<0.01),vWF明显降低(P<0.05)。 结论: 补阳还五汤能够改善MetS模型的胰岛素抵抗和降低vWF水平;方中黄芪是改善胰岛素抵抗的有效成分,6味活血药是降低vWF的有效成分。

Abstract:

Objective: To study the interventive affect and mechanism of Buyang Huanwu decoction (BYHW decoction) on von Willebrand factor (vWF) of animal with metabolic syndrome(MetS). Method: Rats were distributed randomly into 4 groups: empty control, model control, BYHW decoction group,BYHW decoction without Astragali Radix group. To set up pathological modal of MetS, rats were given streptozotocin (STZ) and feed with high-fat and high-carbohydrate diet. Dose: BYHW decoction group 7.02 g ·kg^-1 ig,BYHW decoction without Astragali Radix group 1.07 g ·kg^-1 ig. Both were once a day. Experiment lasts for 8 weeks. Fasting blood glucose (FBG), insulin(Ins), homeostasis model assessment-insulin resistance(HOMA-IR) and vWF in serum were observed. Result: Compared with blank control, Ins, HOMA-IR and vWF were higher in model group (P<0.05). Compared with model, BYHW decoction group was lower in Ins, HOMA-IR and vWF(P<0.05); BYHW decoction without Astragali Radix group was higher in FBG (P<0.01), no evident differences in Ins, HOMA-IR, and lower in vWF. Conclusion: BYHW decoction might prevent IR and reduce vWF. Astragali Radix is the effective part in preventing IR and six other drugs may reduce vWF.