金烫宝烧烫伤喷雾剂治疗烧烫伤创面愈合的研究

Experiment Study on Wound Healing Mechanisms of Jintangbao Spray for Treating Burn Injury

摘要:

目的: 观察金烫宝烧烫伤喷雾剂对大鼠浅Ⅱ度烫伤模型的治疗作用以及其抗炎镇痛作用。 方法: 采用SD大鼠浅Ⅱ度烫伤和KM小鼠足部烫伤创面模型评价其治疗烧烫伤作用;采用角叉菜胶致SD大鼠足肿胀和甲醛致KM小鼠疼痛模型来评价其抗炎镇痛作用。以上动物试验在造模同时分别皮肤涂抹不同质量浓度(0.59,0.39,0.20 g ·mL^-1)的金烫宝烧烫伤喷雾剂和阳性药物,模型组动物皮肤涂抹等容量的赋形剂。 结果: 金烫宝烧烫伤喷雾剂1.76 g/只能明显促进浅Ⅱ度烫伤大鼠模型皮肤创面的愈合,病理组织检查结果显示能够促进浅Ⅱ度烫伤大鼠皮肤表皮损伤和真皮胶原纤维束排列紊乱的修复,促进真皮毛囊增生,减轻炎症细胞对烫伤皮肤的浸润,对烫伤皮肤具有明显的修复作用;金烫宝烧烫伤喷雾剂0.12 g/只和0.08 g/只能明显减少小鼠实验性烫伤足部组织含水量;金烫宝烧烫伤喷雾剂0.70 g/只对角叉菜胶所致大鼠足肿胀有明显的抑制作用;金烫宝烧烫伤喷雾剂0.12 g/只对甲醛引起的小鼠疼痛具有明显镇痛作用。 结论: 金烫宝烧烫伤喷雾剂具有促进烧烫伤创面愈合和抗炎镇痛作用。

Abstract:

Objective: To investigate the wound healing effect of Jintangbao Spray for promoting superficial second degrees burn, as well as anti-inflammatory and analgesic actions. Method: The effect of wound healing was investigated through superficial second degrees burn model in rats and feet burn model in mice. Anti-inflammatory effect was investigated through carrageenan-induced swelling of rat toes. Analgesic effect was investigated through formaldehyde-induced pain in mice. Result: Compared with the model group, Jintangbao Spray could remarkly promote healing of cutis in the trauma with superficial second degrees burn in rats. The pathomorphological evidences of hepatic tissue showed thatJintangbao Spray could remarkably repair cutaneous epidermis injury and the disorganized collagen fiber-bundle of dermis, and promote dermal follicle proliferation, as well as lighten inflammatory cell intiltration. It could significantly reduce water content in the feet burn model in mice, inhibit the carrageenan-induced swelling of rat toes and lighten the pain caused by formaldehyde in mice. Conclusion: Jintangbao Spray can remarkly promote healing of cutis in the trauma and has anti-inflammatory and analgesic effects, and the evidences provide the basis for clinical application of the medicine.