萸连巴布膏抗消化性溃疡的药效学研究

Pharmacodynamics of Yulian Cataplasm for Treatment of Peptic Ulcer

摘要:

目的: 以镇痛、抗炎及抗消化性溃疡为药理指标, 对萸连巴布膏的药效学进行评价,为萸连巴布膏的临床应用提供实验依据 。 方法: 幽门结扎法建立大鼠胃溃疡模型;角叉菜胶致炎法致大鼠足肿胀;化学刺激法造小鼠镇痛抗炎模型;分别观察外用萸连巴布膏高、中、低3个剂量组(大鼠12,6,3 g ·kg^-1,小鼠6,3,1.5 g ·kg^-1)ig对溃疡指数、溃疡抑制率及发生率、足肿胀度、扭体次数及毛细血管通透性的影响。 结果: 外用萸连巴布膏降低溃疡指数,呈剂量依赖性,仅高、中剂量组升高胃蛋白酶的排出量,降低溃疡发生率。萸连巴布膏高、低剂量组能明显降低大鼠足肿胀度,中剂量组作用时间持续时间较长。高、中剂量组均降低毛细血管通透性,高、中、低剂量组均能减少扭体次数,与赋形剂组比较均有显著性差异(P<0.05,P<0.01)。 结论: 萸连巴布膏具有明显的抑制胃溃疡,镇痛抗炎和消肿的作用,为探索经皮给药治疗胃肠疾病的可行性提供了实验依据。

Abstract:

Objective: Analgesic,anti-inflammatory and anti-ulcer were choosed as pharmacological targets to evaluate the pharmacodynamics of Yulian Cataplasm therefore to provide the experimental basis for clinical applications of the cataplasm. Method: The rat experimental gastric ulcer was established by using pyloric ligation. Rat paw edema was caused by inflammation action of carragenan. The mouse model of analgesia was established by the method of somatic wrenching. The effects of Yulian Cataptosm(rats 12,6,3 g ·kg^-1, mice 6,3,1.5 g ·kg^-1,ig)were evaluated by ulcer index, inhibition rate and the incidence of ulcers, foot swelling, writhing and capillary permeability. Yulian cataplasm of high,middle and low dose groups were used respectively. Result: The external Yulian Cataplasm lowered ulcer index in a dose dependent manner. only the high and middle dose groups could increase excretion of pepsin while reduced the incidence of ulcers. Both middle and low dose groups could reduce rat paw swelling, middle dose group lasted longer duration of action. High and middle dose groups could decrease capillary permeability, all the three dose groups could reduce the writhing, and compared with excipient group, there was significant difference (P<0.05, P<0.01). Conclusion: Yulian Cataplasm could obviously inhibite the gastric ulcer, pain and swelling with the role of anti-inflammatory, it also provided an experimental basis on the feasibility of treating gastrointestinal diseases with transdermal drug delivery.