熄风静宁汤对小鼠免疫功能的影响

Effects of Xifeng Jingning Tang on Immunologic Function in Mice

摘要:

目的: 研究熄风静宁汤对免疫低下小鼠免疫功能的影响。 方法: 环磷酰胺50 mg ·kg^-1 ip建立免疫功能低下小鼠模型,熄风静宁汤(3,6,12 g ·kg^-1) ig连续7 d,用碳粒廓清法观察小鼠单核巨噬细胞吞噬功能,耳肿增重法观察小鼠迟发型超敏反应,溶血素抗体测定法观察小鼠溶血素抗体生成。 结果: 熄风静宁汤高、中剂量能明显提高免疫功能低下小鼠的碳粒廓清指数和吞噬指数,拮抗环磷酰胺诱导的免疫低下小鼠耳肿胀度减轻,高剂量能提高免疫低下小鼠溶血素抗体生成。 结论: 熄风静宁汤对小鼠的非特异性免疫、特异性细胞免疫和体液免疫反应有促进作用。

Abstract:

Objective: To study the effect of Xifeng Jingning Tang on immunologic function in mice with immunodepression. Method: The immunodepression mice model was induced by intraperitoneal injection of cyclophosphamide with 50 mg ·kg^-1. Mice were administrated with Xifeng Jingning Tang at doses of 3,6,12 g ·kg^-1 for 7 days.The phagocytic activity of monocytes, the delayed hypersensitivity reaction and the haemolysin antibody formation in mice were observed by means of carbon particle clearance,ear swelling and haemolysin antibodies respectively. Result: The doses of 6 and 12 g ·kg^-1 ·d of Xifeng Jingning Tang could improve immune function in mice shown by carbon clearance index, phagocytic index and ear swelling. Xifeng Jingning Tang 12 g ·kg^-1 could increase content of haemolysin antibody induced by cyclophosphamide. Conclusion: Xifeng Jingning Tang can improve non-specific immunity,and enhance specific cellular immunity and humoral immune function in immunodepression mice induced by cyclophosphamide.