当归散对正常和缩宫素处理大鼠离体子宫活动力的影响

Effects of Danggui San on Isolated Smooth Muscle of Uterus and Uterine Smooth Muscle Strips Stimulated by Oxytocin in Rats

摘要:

目的: 探索复方当归散对大鼠体外子宫平滑肌的作用及部分作用机制。 方法: 运用PowerLab10T生物信号记录系统,记录大鼠体外子宫平滑肌的活动,采用振幅、频率/周期、基线和活动力变化为观察指标,探讨当归散水提取物对子宫平滑肌自主收缩运动的影响,初步探索其治疗先兆流产的作用机制。 结果: 当归散水提取物能明显抑制子宫平滑肌的收缩振幅和基线,显著降低其活动力,均具有统计学意义。缩宫素能明显抬高大鼠离体子宫平滑肌收缩的基线并且缩短收缩周期/增加收缩频率,差异有显著性意义;中药当归散可使缩宫素引起的变化恢复至离体子宫正常收缩水平,使收缩波形的振幅、频率/周期和基线以及活动力与正常离体子宫平滑肌收缩各项指标无显著性差异。 结论: 当归散水提取物能抑制大鼠体外子宫平滑肌自主收缩,并拮抗缩宫素引起的兴奋性收缩,为探索当归散安胎作用机制提供了客观依据。

Abstract:

Objective: To investigate the effects of Danggui San on uterine smooth muscle in rats and the related mechanism of it. Method: The effect of Danggui San decoction on the spontaneous activity of uterine smooth muscle by was observed PowerLab 10T biological signaling system and the activity of isolated uterus smooth muscle was recorded. The indexes include the amplitude, frequency / period, baseline and mobility in rat’s uterine smooth muscle movement track. Result: The water extracts of Danggui San could significantly restrain the amplitude,baseline and mobility; oxytocin could significantly drive up the baseline and increase the frequency. Danggui San could rectify these changes back to the normal level. Conclusion: The water extracts of Danggui San can restrain the mobility of uterine smooth muscle and rectify the excitation of oxytocin. This can afford impersonal evidence for illustrating Danggui San for stabilizing embryo and the partial mechanism involved.