均匀设计法用于乳腺增生动物模型复制的研究

Animal Model Replication of Proliferative Breast by Homogeneous Design 1

摘要:

目的: 规范雌二醇联合黄体酮的乳腺增生造模方法。 方法: 引入均匀设计方法,通过不同实验方案复制乳腺增生动物模型,测量模型大鼠乳头直径和高度,乳腺组织HE染色,镜下观察乳腺导管数目、乳腺导管上皮组织层数及小叶腺泡数,评价造模程度。 结果: 与空白组比较,各实验组动物的乳头直径和高度均有明显增长,乳腺组织切片均有典型的乳腺增生现象,其中以第5,6组最明显。 结论: 制模最佳条件为苯甲酸雌二醇0.42~0.5 mg ·kg,注射时间25~35 d,黄体酮剂量3~4.5 mg ·kg^-1,注射时间为2~3 d,能达到较好的造模效果。

Abstract:

Objective: To investigate the methods in copying the animal proliferative breast model. Method : We copied the animal proliferative breast model with different experimental programs,according to the theory of homogeneous design. And we evaluated varying degrees of success of these models with such indexes as the diameter of the mammilla, height of the mammilla and pathological dye of the galactophore. Result : Compared with the blank control group, the experimental group of animals’ nipple diameter and height were significantly increased in the breast tissue hyperplasia of mammary glands are typical, with the fifth, sixth group was the most obvious. Conclusion: The optimal conditions for establishing the model appear to be:estradiol benzoate 0.42-0.5mg ·kg^-1, the duration for 25-35 days and progesterone 3-4.5 mg ·kg^-1, intramuscular injection for 2-3 days.