地黄饮子对慢性脑低灌注大鼠学习记忆及海马AChE,ChAT的影响

Influence of Dihuangyinzi on Learning and Memory and AChE, ChAT of Hippocampal in Chronic Cerebral Hypoperfusion Rats

摘要:

目的: 探讨地黄饮子对慢性脑低灌注大鼠学习记忆及海马乙酰胆碱酯酶(AChE)、乙酰胆碱转移酶(ChAT)的影响。 方法: 采用双侧颈总动脉结扎制作大鼠慢性脑缺血模型,通过Morris水迷宫试验测定地黄饮子对慢性脑低灌注大鼠学习记忆功能改善情况,并测定大鼠海马脑组织AChE,ChAT的含量。 结果: 地黄饮子组(10,20 g ·kg^-1)与模型对照组比较,Morris水迷宫试验逃避潜伏期缩短,跨越平台次数增加,海马AChE和ChAT含量显著升高(P<0.05)。 结论: 地黄饮子能改善慢性脑低灌注大鼠学习记忆功能,可能与保护胆碱能系统有关。

关键词:地黄饮子;慢性脑低灌注;乙酰胆碱酯酶;乙酰胆碱转移酶

Abstract:

Objective: To investigate influence of Dihuangyinzi on learning and memory and AChE,ChAT of Hippocampal in chronic cerebral hypoperfusion rats. Method: Using the model of chronic cerebral ischemia by two-vessel occlusion, Learning and memory memory improvement was measured by Morris water maze test in rats of chronic cerebral hypoperfusion of Dihuangyinzi, and AChE,ChAT content were measured in hippocampus of rats. Result: Compared with the control group,Morris water maze test,Dihuangyinzi group escape latency Shortened,the number of cross platform increased,AChE and ChAT Content in the hippocampus were significantly increased. Conclusion: Dihuangyinzi can improve learning and memory function in rats of chronic cerebral hypoperfusion,which may protect the cholinergic system.

Key words:Dihuangyinzi;chronic cerebral hypoperfusion;AChE;ChAT