钩藤与天麻配伍前后异钩藤碱在SHR大鼠肝脏和肾脏的分布

Distribution of Isorhynchophyline in Liver and Kidney of SHR Rats before and after Compatiblity of Unicariae Ramulus Cum Uncis with Gastrodiae Rhizoma

摘要:

目的: 观察钩藤与天麻配伍前后异构藤碱在SHR大鼠肝脏和肾脏的分布变化,探讨药物配伍前后对其归经的影响。 方法: 采用原发性高血压大鼠(SHR),钩藤组给予钩藤水煎液,天麻钩藤组给予钩藤天麻配伍后水煎液,空白组给予生理盐水,给药剂量为1.172 g ·kg^-1,用高效液相色谱法测定钩藤与天麻配伍前后其效应成分异钩藤碱在SHR大鼠肝脏和肾脏中的含量;色谱条件:色谱柱:Waters^TM(4.6 mm×250 mm,RP 18.5 μm);流动相甲醇-水(70 ∶30) pH 8.0;流速为0.8 mL ·min^-1,检测波长254 nm;柱温30 ℃;进样量20 μL。 结果: 异钩藤碱对照品在肝脏和肾脏组织中在0.08~16.0 mg ·L^-1具有较好的线性关系;钩藤与天麻配伍前后在肝脏和肾脏的异钩藤碱的分布具有显著的差异性(P<0.05)。 结论: 中药配伍与归经之间有着密切的相关性。

Abstract:

Objective: To study the distribution of isorhynchophyline in liver and kidney tissues of SHR rats before and after compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Unics Le, to investigate the impact of channel tropism before and after combination. Method: Uncariae Ramulus Cum Uncis decoction group was administrated Uncariae Ramulus Cum Unics,Tianma Gouteng decoction group was administrated with decoction of compatible Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis at a dose of 1.172 g ·kg^-1. A blank group was received normal saline. Then using HPLC to determinate the isorhynchophyline in liver and kidney tissues of SHR rats before and after the compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis. Analytical column was Waters^TM(4.6 mm×250 mm, RP 18.5 μm). The mobile phase consisted of methanol-water (70 ∶30),pH 8.0,rate was 10.8 mL · min^-1. The detector wave length was set at 254 nm,the column temperature was 30 ℃. Result: Isorhynchophyline in liver and kidney tissues was linear in the range of 0.08-16.0 mg ·L^-1. There existed significant (P<0.05) differences in liver and kidney tissues before and after the compatibility. Conclution :The compatibility of Chinese medicine has close correlation with channel tropism.