银屑灵与肿节风中异嗪皮啶的药代动力学比较

Pharmacokinetic Comparison of Isofraxidin after Oral Administration with Yinxieling or Decoction of Sarcandra glabra

摘要:

目的: 建立检测大鼠血清中异嗪皮啶的方法,探讨中药复方银屑灵与单味药肿节风相比较对异嗪皮啶药代动力学参数的影响。 方法: 用银屑灵与肿节风的水煎剂ig给药,用HPCL检测给药后的血清样品中的异嗪皮啶含量,并用DAS(2.0)药代动力学软件对结果进行分析。 结果: 异嗪皮啶的线性范围为0.25~12 mg ·L^-1;检测限0.25 mg ·L^-1;提取回收率70.4%~71.9%(RSD 1.1%)。日内、日间精密度RSD小于8.9%。与肿节风组相比,银屑灵组异嗪皮啶药代动力学参数无显著性差异。 结论: 该方法专属性强、灵敏度高、准确性好;复方中的其他成分不影响异秦皮啶的体内过程。

Abstract:

Objective: To establish the method for analyzing isofraxidin in rat blood serum and investigate pharmacokinetic indexes of isofraxidin after oral administration of Yinxieling or Sarcandra glabra(Thumb.) Nakai. Method: Sprague-Dawley rats were administrated Yinxieling or decoction of S.glabra respectively. Serum concentration of isofarxidin was measured by HPLC. The results were analyzed by pharmacokinetic software DAS(2.0). Result: The linearity of the calibration curve was good in the range of 0.25-12 mg ·L^-1 for isofraxidin and the detection limit for isofraxidin were 0.25 mg ·L^-1.The extraction recoveries of isofraxidin were 70.9%-71.9% with RSD was 1.1%. The intra-day precision and inter-day precision of isofraxidin were lower than 8.9%. The difference of pharmacokinetic parameters between the group of Yinxieling and S.glabra were unremarkable. Conclusion: The method is exclusive,sensitive and accurate. Compound prescription of Chinese medicine did not affect the pharmacokinetics of isofraxidin.