基于化学组分动态变化的附子配伍甘草煎煮条件研究

Optimization of Decoction Conditions of Compatibility of Aconitum carmichaelii and Glycyrrhiza uralensis Base on Dynamic Change of Chemical Components

摘要:

目的: 研究与甘草配伍前后,附子化学组分在煎煮过程中的动态变化规律,以确定附子配伍甘草的最佳煎煮条件。 方法: 测定不同时间附子单煎液和附子-甘草合煎液中乌头总碱及3种双酯型生物碱的含量,确定最佳煎煮条件;考察最佳煎煮工艺所得汤液的大鼠心脏毒性。 结果: 附子-甘草配伍的最佳煎煮条件为大火煮沸后小火微沸保持30 min;在煎煮0~90 min时,附子-甘草配伍前后的乌头总碱及3种双酯型生物碱的含量呈现不同的变化趋势,30 min时达两者综合的峰值;煎煮30 min的单附煎液和附子-甘草合煎液均表现出一定的大鼠毒性,但合煎液心脏毒性较小。 结论: 优选的配伍煎煮工艺可为中药复方及临床应用中附子的"减毒存性"提供参考。

Abstract:

Objective:To study on dynamic change rule of chemical components from Aconitum carmichaelii during decoction process after and before compatibility with Glycyrrhiza uralensis,in order to determine optimum decoction conditions of compatibility of G.uralensis and A. carmichaelii. Method: To determine optimum decoction conditions by determining the contents of total alkaloids and 3 kinds of double ester alkaloid in different decoction time,which were from single decoction liquid of A. carmichaelii and mixed decoction liquid of G.uralensis and A.carmichaelii.And rats cardiac toxicity of soup with optimum boiling technology was investigated. Result: Optimum boiling technology of G. uralensis-A. carmichaelii was:after boiling with a blaze,kept boiling 30 min with small fire.During 0-90 min decoction process,it showed different change trend of the contents of total alkaloids and 3 kinds of double ester alkaloid before and after compatibility of G. uralensis and A. carmichaelii,it reached to both comprehensive peak value in 30 min;both single decoction liquid of A. Carmichaelii and mixed decoction liquid of G. uralensis and A. carmichaelii showed some cardiac toxicity on rats in boiling 30 min,but cardiac toxicity of the latter was relative less. Conclusion: Optimized compatibility boiling technology could provide reference for 'reducing toxicity and storing efficacy’of A. carmichaelii in clinical application and traditional Chinese medicine compound.