巴戟天低聚果糖树脂法脱色的工艺考察

Investigation of Decolorization Process for Fructo-oligosaccharide from Morinda officinalis by Resin Adsorption

摘要:

目的: 考察树脂法对巴戟天低聚果糖中所含色素的脱除能力。 方法: 以脱色率、寡糖保留率为指标,筛选合适树脂对巴戟天低聚果糖进行脱色,采用单因素试验考察其静态及动态吸附工艺。HPLC-ELSD测定耐斯糖含量。 结果: 确定采用D941型树脂,其最佳工艺条件为室温(25 ℃)下,15 g·L^-1巴戟天寡糖溶液(pH 5.88)4 BV,流速4 BV·h^-1,径高比1∶10。脱色率92.54 %,寡糖保留率82.76 %,耐斯糖质量分数由22.46 %升高至29.94 %。 结论: D941型树脂对巴戟天低聚果糖具有良好的脱色能力。

Abstract:

Objective:To study on elution property of pigment contained in fructo-oligofructose from Morinda officinalis suitable by resin adsorption. Method: With decolorization rate and retention rate of oligosaccharide as indexes, suitable resin was chosen,and static and dynamic adsorption tests were carried out to investigate decolorization effect of fructo-oligofructose from M. officinalis by single factor test.The content of nystose was determined by HPLC-ELSD. Result: D941 resin was chosen,optimum technology conditions were as follows:room temperature(25 ℃),oligosaccharide solution 4 BV(pH 5.88,15 g·L^-1), flow rate 4 BV·h^-1,diameter high ratio 1∶10.Under these conditions, decolorization rate and retention rate of oligosaccharide were 92.54%,82.76%,respectively,and the content of nystose increased from 22.46% to 29.94%. Conclusion: D941 resin had good decolorization effect on fructo-oligofructose from M. officinalis.