白花蛇舌草中总三萜酸的提取纯化工艺优选

Optimization of Extraction and Purification Technology for Total Triterpene Acid from Hedyotis diffusa

摘要:

目的: 优选白花蛇舌草中总三萜酸的提取纯化工艺。 方法: 以总三萜酸提取率为指标,选取乙醇用量、乙醇体积分数及回流时间为考察因素,采用正交试验优选白花蛇舌草中总三萜酸的提取工艺;通过静态吸附和解吸试验筛选树脂型号,采用单因素试验考察总三萜酸的大孔树脂纯化工艺。 结果: 最佳提取工艺为加10倍量70%乙醇回流提取1.5 h。采用AB-8型大孔树脂,其纯化工艺为上样流速2 BV·h^-1,上样液pH 5,上样液质量浓度0.25 g·L^-1,洗脱剂pH 7,洗脱乙醇体积分数80%,洗脱速度2 BV·h^-1。 结论: 优选的工艺操作简单、稳定可行、重复性好,可推广于工业化生产应用。

Abstract:

Objective:To optimize extraction and purification technology of total triterpene acid from Hedyotis diffusa. Method: With extraction rate of total triterpene acid as index,the amount and concentration of ethanol,reflux time were chosen as factors,extraction technology of total triterpene acid from H. diffusa was optimized by orthogonal test;Resin model was screened by static adsorption and desorption test,purification technology of total triterpene acid was investigated by single factor test. Result: Optimum extraction technology was:reflux extracted 1.5 h with 10-fold 70% ethanol.Optimum purification technology of AB-8 macroporous resin was:sample flow rate 2 BV·h^-1,the concentration of sample liquid 0.25 g·L^-1, pH 5,the concentration of elution was 80% ethanol,eluting velocity 2 BV·h^-1 with pH 7. Conclusion: Optimized technology was simple,stable with good reproduciblity,which was suitable for industrial production of total triterpene acid.