响应面法优化油腻型痤疮方中药配比

Response Surface Method for Optimizing Ratio of Oily-acne Herbs Proportioning

摘要:

目的: 通过响应面优化方法得到中医验方油腻型痤疮方的最佳复配比例。 方法: 采用体外抑菌实验、体外抑制透明质酸酶和凝血酶实验、抑制人体皮脂分泌实验,以这些实验结果为指标,进行响应面优化设计,最终找到最佳配比。 结果: 模拟预测最佳中药组方的复配比例是:蒲公英提取物1.19份,厚朴提取物2.19份,川芎提取物2.24份,在此复配比例下可得到相对抑脂率的理论值为38.9%。由于3种中药提取物都是0.2 g·mL^-1,因此提取物的比例可以表示为它们中药材本身的质量比。实验实际检测值为40.25%。 结论: 用响应面优化法模拟预测的抑脂率与实测值非常相似,响应面优化法可较好地用于复方配伍比例的研究。

Abstract:

Objective:To optimize mixed proportion for prescription of oily acne herbs through response surface method. Method: Bacteriostasis experiment, hyaluronic acid and inhibiting thrombin experiment in vitro and inhibiting human sebum secretion experiment were carried out. Based on the results of these experiments, the indicators were analyzed by response surface method to find the best ratio finally. Result: Simulation and prediction of the best ratios of the herb extracts showed dandelion extract (1.19), Magnolia bark extract (2.19) and ligusticumchuanxiong extract (2.24). The prediction estimated a relative-inhibiting compound fat rate of 38.9%. Because all three kinds of Chinese traditional medicine extract is 0.2 g·mL^-1, extracts can be expressed as a proportion of their own quality of Chinese herbal medicines. The real experimental value of the relative-inhibiting compound fat rate was 40.25%. Conclusion: The predicted value of the relative-inhibiting compound fat rate though response surface method is very similar to the experimental value. Response surface method can be employed in the proportion study of compound prescriptions.