Abstract:Objective:To study chemical constituents from Isatis indigotica. Method: Chemical constituents were isolated by many chromatographic separation method,and structure of these were identified by purified by1H,13C-NMR and other spectroscopy technology and physicochemical data. Result: Six compounds were isolated and identified as n-heneicosanoic acid, β-sitosterol, indirubin, adenosine, emodin, Fructopyrano-(1→4)-glucopyranose. Conclusion: N-heneicosanoic acid and Fructopyrano-(1→4)-glucopyranose were first isolated from I. indigotica.