微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较

Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Chrysanthemum indicum Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method

摘要:

目的: 建立微波协助提取法提取野菊花中有效成分蒙花苷含量测定方法,比较微波协助提取法与药典法提取蒙花苷优势。 方法: 采用Dikma Diamonsil C₁₈色谱柱(4.6 mm×150 mm,5 μm),流动相甲醇-水-冰醋酸(26∶23∶1),检测波长334 nm,柱温30 ℃,流速1 mL·min^-1。 结果: 微波提取时间15 min,提取温度100 ℃,提取溶剂50%乙醇。蒙花苷在0.04~1.2 μg呈良好线性关系(r=0.999 9),平均回收率100.13%(n=6)。 结论: 微波提取法较药典法提取蒙花苷更简便快速、准确,为一种符合环保绿色理念的含量测定方法,可用于检测野菊花饮片中蒙花苷含量。

Abstract:

Objective:To establish a microwave-assisted extraction method for the extraction of the linarin from Chrysanthemun indicum. Comparison of the determination of linarin from C. indicum between microwave-assisted extraction and Chinese pharmacopeia method was made. Method: Dikma Diamonsil C₁₈ column(4.6 mm×150 mm,5 μm)was used. The mobile phase cousisted of methanol-water-acetic acid (26∶23∶1).The temperature of column was kept at 30 ℃. The flow rate was 1.0 mL·min^-1 and the detection wave-length was at 334 nm. Result: Microwave extractive time was 15 min,the temperature was 100 ℃,the extraction solvent was 50% ethanol. The calibration curve was in good linearity within the range from 0.04 to 1.2 μg(r=0.999 9). The average recovery was 100.13%(n=6). Conclusion: The method is simple,rapid,as well as precise and reliable,and can be used for the determination of linarin from C. indicum.