HPLC测定湿疹纳米乳喷雾剂中丹皮酚的含量

Determination of Paeonol Concentration in Eczema Nanoemulsion Spray by HPLC

摘要:

目的: 建立湿疹纳米乳喷雾剂中丹皮酚的HPLC定量方法,控制该制剂的质量。 方法: 采用高效液相色谱法,Phenomenex C₁₈-ODS色谱柱,流动相甲醇-水(55∶45),流速1.0 mL·min^-1,检测波长274 nm。 结果: 标准曲线线性范围在10.0~100.0 mg·L^-1(r＝0.999 9),48 h内稳定,加样回收率99.60%,RSD 1.123%。理论塔板数均在6 000以上。分离度达1.5以上。 结论: 方法简便、快速、灵敏、准确,稳定性及重复性好,可作为该制剂中丹皮酚含量测定的常规方法。

Abstract:

Objective:To establish a method for the determination of paeonol in Eczema Nanoemulsion spray by HPLC. Method: Using HPLC technique,we detected the samples by the C₁₈ ODS column of Phenomenex company under the wave length at 274 nm, the chromatographic conditions were phenomenex C₁₈ -ODS column, mobile phase of methanol-water (55∶45), flow rate of 1.0 mL·min^-1 and detection wave-length at 274 nm. Result: The standard curve was linear over the range of 10.0-100.0 mg·L^-1,with the correlation coefficient of 0.999 9.It is steady in 48 hour. The average recovery was 99.70% with RSD 1.12%(n=9). The theoretical plates are above 6 000,separating degree is greater than 1.5. Conclusion: The analytical method is simple,rapid,sensitive,accurate; stability and reproducibility are good,and the method could be used to determine content of paeonol in a Chinese medicine Eczema Nanoemulsion spray.