小飞蓬药材薄层鉴别与含量测定

Identification and Determination of Erigeron canawdensis

摘要:

目的: 探讨小飞蓬药材定性、定量检测方法。 方法: 采用TLC鉴别小飞蓬中的槲皮素,HPLC测定小飞蓬中槲皮素的含量。 结果: TLC以正己烷-乙酸丁酯-甲酸(6∶5∶1) 为展开系统,可以清晰地检测槲皮素; HPLC采用日本岛津Shimadzu C₁₈(4.6 mm×250 mm, 5 μm)色谱柱,流动相-甲醇-0.4%磷酸(47∶53),检测波长257 nm,流速1 mL·min^-1,柱温30 ℃,槲皮素在0.504~1.134 μg呈良好线性关系,平均回收率分别为97.97%%,RSD分别为1.2 %。 结论: 该法可作为小飞蓬药材的定性、定量检测方法。

Abstract:

Objective:To discuss detection method of the qualitative and quantitative of Erigeron canawdensis. Method: TLC was used to determine quercetin of E. canawdensis. The content of quercetin was determined by HPLC. Result: n-Hexane-butyl acetate-formic (6∶5∶1) is used as TLC deployment system. It may clearly detect quercetin; Shimadzu C₁₈ (4.6 mm×250 mm, 5 μm) column was used in HPLC with mobile phase methanol-0.4% phosphoric acid (47∶53). The detection wavelength was set at 257 nm and the flow rate was 1 mL·min^-1; the column temperature was at 30 ℃.The linear range of quercetin were respectively 0.504-1.134 μg, and their average recovery were respectively 97.97%,RSD were respectively 1.2%. Conclusion: This method is used as detection method of the qualitative and quantitative of E. canawdensis.