HPLC同时测定肝尔舒微丸中3种有效成分的含量

HPLC Simultaneous Determination of Three Active Components in Ganershu Pellets

摘要:

目的: 建立HPLC同时测定肝尔舒微丸中的槲皮素、山奈素和异鼠李素含量的方法。 方法: 色谱柱为Hedera ODS-2柱(4.6 mm×200 mm,5 μm),流动相0.4%磷酸溶液(A)-甲醇(B)梯度洗脱,检测波长360 nm,流速1 mL·min^-1,柱温30 ℃。结果槲皮素、山奈素和异鼠李素分别在0.042 8~0.428,0.003 49~0.034 9,0.041 8~0.418 μg呈良好的线性关系,r分别为0.999 8,0.999 1,0.999 2;平均加样回收率槲皮素99.88%,RSD 0.77%(n=6);山奈素为99.44%,RSD 1.2%;异鼠李素为97.50%,RSD 1.5%(n=6)。 结论: 该法结果准确,重复性好,可用于肝尔舒微丸的质量控制。

Abstract:

Objective:To establish a method of determining quercetin, kaempferide and isorhamnetin in Ganershu pellets by HPLC. Method: An Hedera ODS-2 column(4.6 mm×200 mm,5 μm)was used at 30 ℃ with the mobile phase of 0.4% phosphate (A) and methanol (B)by gradient elution.The detection wavelength was set at 360 nm and the flow rate was 1.0 mL·min^-1. Result: The quercetin, kaempferide and isorhamnetin had good linear relationship within the ranges of 0.042 8-0.42 8 μg(r=0.999 8), 0.003 49-0.034 9 μg (r=0.999 1),0.041 8-0.418 μg(r=0.999 2).The average recoveries were 99.88%,99.44% and 97.50% with RSD 0.77%(n=6),1.2%(n=6),1.5%(n=6) respectively. Conclusion: The method was accurate and reproducibility was good. The method can be used for the quality control of Ganershu pellets.