HPLC测定樟木中黄樟醚二醇的含量

Determination of Safrolglycol in Cinnamomum camphora by HPLC

摘要:

目的: 建立樟木中主要成分黄樟醚二醇的含量测定方法。 方法: 采用C₁₈(4.6 mm×200 mm,5 μm)色谱柱;流动相乙腈-水(14∶86),检测波长282 nm,柱温30 ℃。 结果: 该成分在进样量1.014~5.07 μg与峰面积线性关系良好(r=0.999 9),平均回收率为99.0%,1.89% (n=6)。 结论: 该方法测定樟木中主要成分黄樟醚二醇的含量,方便、准确、灵敏度高,重复性好,可适用于樟木药材的质量控制。

Abstract:

Objective:To establish a method for the determination of safrolglycol in Cinnamomum camphora. Method: Using HPLC with Hypersil ODS column, CH₃CN-H₂O (14∶86) as mobile phase and detection wavelength at 282 nm. Result: The linearity of the calibration curve was evaluated over the amount range of 1.014-5.07 μg for safrolglycol, and good linearity with correlation coefficient (r) of 0.999 9 was obtained. The recovery and RSD were 99.0% and 1.89% (n=6) respectively. Conclusion: This method is rapid, simple and accurate, thus may be used for the quality control of C. camphora.