二氢卟吩e6微透析回收率的体外测定方法及影响因素研究

Study on the Relative Recovery of Chlorine-e6 in Microdialysis Probe and its Influence Factors

摘要:

目的: 建立二氢卟吩e6微透析探针相对回收率(relative recovery,RR)的测定方法,并考察流速、浓度、温度、时间对RR的影响,为体内微透析研究提供实验依据。 方法: 利用浓度差法(增量法、减量法)进行二氢卟吩e6微透析探针体外回收率的比较研究,采用高效液相色谱仪(HPLC)测定二氢卟吩e6微透析取样后的浓度,利用探针回收率公式计算探针的RR。 结果: 在同一浓度下,两种方法测定RR均随流速的增加而减少;增量法和减量法测得的RR一致;在同一流速下,浓度对RR的影响不大;RR在12 h内保持稳定;RR随温度的上升明显增加。 结论: 确定了影响微透析体外回收率的因素,利用减量法测定的探针回收率与增量法接近,反向透析法可作为体内微透析研究中的二氢卟吩e6回收率的测定方法。

Abstract:

Objective:To establish a method for the relative recovery (RR) of chlorine-e6 in microdialysis probe and to investigate the effect of the concentration,time and temperature on RR in chlorin-e6 microdialysis probe. Method: Gain method and loss method were used for the study. The concentration of chlorine-e6 in micro-dialysate samples was detected by HPLC and calculated probe RR with the formula used for the probe. Result: At the same concentration, RR decreased with the increase of the flow rate in the two methods. RR detected by gain method was similar to that detected by loss method. At the same flow rate, the concentration had no obvious effect on RR. RR kept steady within 12 hours, and increased obviously with the temperature. Conclusion: The influencing factors on RR in chlorin-e6 microdialysis probe are confirmed. The probe recovery obtained by gain method is similar to that by loss method. Retrodialysis can be used for the determination of the chlorine-e6 in-vivo recovery.