浮小麦药材质量控制研究

Criteria of Quality Control for Triticum aestivum

摘要:

目的: 建立浮小麦药材的质量控制方法。 方法: 对浮小麦药材的性状鉴别、显微鉴别等方面进行生药学的研究;采用薄层色谱法(TLC)对浮小麦中5-二十一烷基间苯二酚进行定性鉴别;采用高效液相色谱法建立浮小麦药材中5-二十一烷 基间苯二酚的含量测定方法。 结果: 对浮小麦的性状、显微特征进行了描述;薄层鉴别的色谱斑点清晰,特征性强,重复性好;在选定的高效液相色谱条件下5-二十一烷基间苯二酚同其他成分达到较好的基线分离,5-二十一烷基间苯二酚在129.5~259 0 ng 呈良好的线性关系,Y=5 056.5X+201.5(r=0.999 9);平均回收率为101.26%,RSD 1.89%。 结论: 建立的方法简单易行、重复性好、结果准确,能有效鉴别和评价浮小麦药材,可作为浮小麦药材的质量控制方法。

Abstract:

Objective:To establish the quality control methods for Triticum aestivum. Method: The pharmacognostical methods were employed for the morphological and histological characters of Triticum aestivum. TLC was used to identify 5-heneicosyl resorcinol and the content of 5-heneicosyl resorcinol was determined by HPLC. Result: The morphological and histological characters of Triticum aestivum were observed. The spots were observed in the TLC with high characterisitic and good repeatability. Under the selected HPLC method, 5-heneicosyl resorcinol was well separated with others. The linear range of 5-heneicosyl resorcinol was 129.5-2 590 ng. Y=5 056.5X+201.5(r=0.999 9).The average recovery of 5-heneicosyl resorcinol was 101.26%, and the RSD was 1.89%. Conclusion: The established method is simple, feasible, reproducible and accurate. It is suitable for identification and evaluation of the quality, and can be used as the quality control standard of Triticum aestivum.