人参茎叶水提液中人参皂苷热稳定性研究及其有效期推算

Studies on Thermal Stability of Ginsenosides Aqueous Solution from the Stem and Leaf of Ginseng and Calculate the Term of Validity

摘要:

目的: 研究水煎煮提取过程中加热时间、温度对人参茎叶水提液中人参皂苷的影响及受热后人参皂苷含量的变化情况,并且推算人参茎叶水提液的有效期。 方法: 采用高效液相色谱法测定不同加热条件下人参茎叶水提液中人参皂苷Rg₁,Re的含量(Agilent Extend-C₁₈柱(4.6 mm×250 mm,5 μm),流动相乙腈-水(19∶81),洗脱程序:等度40 min,检测波长203 nm,流速1.0 mL·min^-1, 柱温30 ℃,并以经典恒温法推算其有效期。 结果: 2种人参皂苷在不同温度下加热12 h,均会发生不同程度的降解反应,其中,100 ℃条件下降解速率最快,80 ℃次之,60 ℃相对较慢,分别降至约初始含量的20%、60%、80%,以lgK对1/T进行一元线性回归,得回归方程,人参皂苷Rg: lgK=-2 396.2/T+5.587 3,人参皂苷Re:lgK=-2 518.2/T+5.901 6,通过回归方程计算人参茎叶水提液中人参皂苷Rg1,Re的有效期分别为30.1和37.6 h。 结论: 人参茎叶水提液中人参皂苷Rg₁,Re的含量,随加热时间延长而不断下降,温度越高,下降速率越快;人参茎叶水提液的有效期较短,故含人参茎叶水提液的制剂中间体应在其有效期内完成制剂。

Abstract:

Objective:The influence of heating time and temperature on the content of ginsenosides from stems and leaves of ginseng during water decoction process was researched to calculate the term of validity. Method: The HPLC was used to determine the content of ginsenoside Rg₁ and ginsenoside Re extracted in different heating conditions.An Agilent Extend-C₁₈ column(4.6 mm×250 mm, 5 μm) was used with acetonitrile-water(19∶81)as the mobile phase at the flow rate of 1.0 mL·min^-1,and the column temperature was maintained at 30 ℃ and the detection wavelength was set at 203 nm. The term of validity was caculated by classical homeothermia. Result: When the heat comes to 12 h, ginsenoside Rg₁ and ginsenoside Re had different degree of degradation, and they degraded rapidly when temperature is at 100 ℃. It’s only 20 percent of the initial content at 100 ℃,and 60% at 80 ℃, 80% at 60 ℃.With simple linear regression analysis to 1/T as lgK, the regression equations are:lgK=-2 396.2/T+5.587 3 for ginsenoside Rg1, lgK=-2 518.2/T+5.901 6 for ginsenoside Re. The term of validity of ginsenoside Rg₁ and ginsenoside Re were calculated as 30.1 h and 37.6 h. Conclusion: The content of ginsenoside Rg₁ and ginsenoside Re decreased with time increasing and temperature increasing. The term of validity about stems and leaves of Panax ginseng is in short period.So,intermediate which contains water extract of stems and leaves of P. ginseng should accomplish the preparation within the term of validity.