北细辛不同提取物中总马兜铃酸的含量测定

Determination of Total Aristolochic Acid in Different Extracts of Asarum heterotropoides

摘要:

目的: 测定北细辛不同提取物中总马兜铃酸的含量,为其临床应用提供实验依据。 方法: 以水、乙酸乙酯、30%乙醇、50%乙醇、70%乙醇、95%乙醇提取北细辛药材,以马兜铃酸A为对照,采用紫外分光光度法,检测波长为390 nm。 结果: 马兜铃酸A含量在3.0~30.0 mg·L^-1与吸收度呈良好线性关系, Y=19.844X+0.027 2(r=0.999 6),平均回收率为98.51%,RSD 1.32%。北细辛不同提取物中总马兜铃酸的含量为1.34%~3.15%,以水提取物含量最低,95%乙醇提取物含量最高。 结论: 方法测定结果准确可靠,重复性好,可用于北细辛提取物中总马兜铃酸的含量测定。

Abstract:

Objective:To determine the content of total aristolochic acid in different extracts of Asarum heterotropoides, and provide the basis for clinical application of A. heterotropoides. Method: A. heterotropoides was extracted with water, ethyl acetate, 30% ethanol, 50% ethanol, 70% ethanol and 95% ethanol as extracting solvent. The ultraviolet spectrophotometry was used with aristolochic acid A as the chemical reference. The UV detector wavelength was set at 390 nm. Result: The content of aristolochic acid A showed a linear relation with the spectrophotometric absorbance within the range of 3.0-30.0 mg·L^-1, and the linear equation was Y=19.844X+0.027 2 (r=0.999 6). The average recovery was 98.51%, and RSD was 1.32%. The total aristolochic acid content varied from 1.34%-3.15%. It was the lowest in the water extract and highest in the 95% ethanolic extract. Conclusion: The method is reliable, accurate and suitable for determining the content of the total aristolochic acid in extracts of A. heterotropoides.